@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003621, author = "Sun, Tianyu and Cao, Peng and Sun, Kexin and Li, Chenxu and Jiang, Mengqi and Jia, Weiqi and Wang, Xiangjing and Zhao, Junwei and Xiang, Wensheng", title = "Agromyces tardus sp. nov., an actinobacterium isolated from the rhizosphere soil of wheat (Triticum aestivum L.)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "10", pages = "3268-3275", doi = "https://doi.org/10.1099/ijsem.0.003621", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003621", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "polyphasic analysis", keywords = "Agromyces tardus sp. nov.", keywords = "16S rRNA gene", abstract = "A Gram-stain-positive, aerobic, heterotrophic, non-spore-forming and rod-shaped strain, designated SJ-23T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Langfang, Hebei Province, central PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Agromyces . The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipid and three unidentified lipids. The predominant menaquinones detected were MK-12, MK-11 and MK-10. Major fatty acids were identified as anteiso-C17 : 0, anteiso-C15 : 0 and iso-C16 : 0. The 16S rRNA gene sequence analysis showed that strain SJ-23T belongs to the genus Agromyces with high sequence similarities to Agromyces ramosus DSM 43045T (99.2 %), Agromycescerinus subsp. cerinus DSM 8595T (98.8 %) and Agromyces cerinus subsp. nitratus DSM 8596T (98.6 %). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a separate branch in the genus Agromyces . Furthermore, the combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain SJ-23T could be distinguished from its closest relatives. Therefore, it is proposed that strain SJ-23T represents a novel species of the genus Agromyces , for which the name Agromyces tardus sp. nov. is proposed. The type strain is SJ-23T (=CGMCC 4.7419T=DSM 105049T).", }