1887

Abstract

A novel strain WCHEs120001 was recovered from the wound secretion of a patient at West China Hospital, Chengdu, PR China, in 2017. The strain was Gram-stain-negative, facultatively anaerobic, motile, and non-spore-forming. A preliminary analysis based on the 16S rRNA gene sequence revealed that this strain was closely related to members of the genus . The strain was subjected to whole genome sequencing. Phylogenetic analysis based on core gene sequences of type strains of all species revealed that this strain belonged to the genus but were distinct from any previously known species. Both average nucleotide identity (ANI) and DNA–DNA hybridisation (isDDH) values between strain WCHEs120001 and type strains of all known species were 82.02 to 92.37% and 25.6 to 50.9 %, respectively, which are lower than the 95 % (ANI) and 70 % (isDDH) cutoff for species delineation. The major fatty acids of the strain WCHEs120001 are C, sum of Cω7/Cω6 and Cω7, which are similar to other species. Genomic DNA G+C content of strain WCHEs120001 was 53.33 mol%. Strain WCHEs120001 is positive for methyl--glucopyranoside but does not ferment adonitol, -arabitol, dulcitol and melibiose, which distinguishes it from all other species. Genotypic and phenotypic characteristics indicate that strain WCHEs120001 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain of sp. nov. is WCHEs120001 (=GDMCC1.1570=NCTC 14272).

Keyword(s): genome , Kosakonia and secretion
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/content/journal/ijsem/10.1099/ijsem.0.003606
2019-07-29
2019-08-25
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