1887

Abstract

During a survey of fungal diversity in a deserted rocky area in Yunnan, PR China, a new species, , was identified. This new species is characterized by having phialidic conidiogenous cells with conspicuous collarettes, and aseptate, verrucose, ellipsoidal to sometimes ovoid, olivaceous brown to dark brown conidia. Morphologically, is similar to , but can be easily distinguished due to its hyaline conidiophores and smaller conidia. Phylogenetic analysis based on DNA sequences at five loci showed that our strain grouped together with and . Here, the new species is described and illustrated, and a key to the species of the genus is provided.

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2019-10-01
2024-04-19
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References

  1. Crous PW, Shivas RG, Quaedvlieg W, van der Bank M, Zhang Y et al. Fungal planet description sheets: 214–280. Persoonia 2014; 32:184–306 [View Article][PubMed]
    [Google Scholar]
  2. Maharachchikumbura SSN, Hyde KD, Jones EBG, Mckenzie EHC, Huang S-K et al. Towards a natural classification and backbone tree for Sordariomycetes. Fungal Divers 2015; 72:199–301 [View Article]
    [Google Scholar]
  3. Maharachchikumbura SSN, Hyde KD, Jones EBG, Mckenzie EHC, Bhat JD et al. Families of Sordariomycetes . Fungal Divers 2016; 79:1–317 [View Article]
    [Google Scholar]
  4. Nag Raj TR. Coelomyceous Anamorphs with Appendage-Bearing Conidia Waterloo, Ontario, Canada: Mycologue Publications; 1993
    [Google Scholar]
  5. Rossman AY, Samuels GJ, Rogerson CT, Lowen R. Genera of Bionectriaceae, Hypocreaceae and Nectriaceae (Hypocreales, Ascomycetes). Stud Mycol 1999; 42:1–248
    [Google Scholar]
  6. Seifert KA, Louis-Seize G, Sampson G. Myrothecium acadiense, a new hyphomycete isolated from the weed Tussilago farfara . Mycotaxon 2003; 87:317–327
    [Google Scholar]
  7. Mel'nik V, Lee S, Groenewald JZ, Crous PW. New hyphomycetes from Restionaceae in fynbos: Parasarcopodium ceratocaryi gen. et sp. nov., and Rhexodenticula elegiae sp. nov. Mycol Prog 2004; 3:19–28 [View Article]
    [Google Scholar]
  8. Decock C, Huret S, Bivort C. Anamorphic fungi from French Guyana. Septomyrothecium sp. nov. and S. setiramosum comb. nov. (anamorphic Hypocreales, Ascomycota). Cryptogamie Mycologie 2008; 29:321–331
    [Google Scholar]
  9. Lombard L, Houbraken J, Decock C, Samson RA, Meijer M et al. Generic hyper-diversity in Stachybotriaceae . Persoonia 2016; 36:156–246 [View Article][PubMed]
    [Google Scholar]
  10. Bisby GR. Stachybotrys and Memnoniella . Trans Br Mycol Soc 1945; 28:11–12 [View Article]
    [Google Scholar]
  11. Jong SC, Davis EE. Contribution to the knowledge of Stachybotrys and Memnoniella in culture. Mycotaxon 1976; 3:409–485
    [Google Scholar]
  12. Smith G. Some new and interesting species of micro-fungi. III. Trans Br Mycol Soc 1962; 45:387–IN2 [View Article]
    [Google Scholar]
  13. Wang Y, Hyde KD, Mckenzie EHC, Jiang Y-L, Li D-W, Dw L et al. Overview of Stachybotrys (Memnoniella) and current species status. Fungal Divers 2015; 71:17–83 [View Article]
    [Google Scholar]
  14. Galloway LD. Note on an unusual mould fungus. Trans Br Mycol Soc 1933; 18:163–166 [View Article]
    [Google Scholar]
  15. Huang Y, Zhao P, Zhang Z, Li X, He C et al. Transpiration of Cyclobalanopsis glauca (syn. Quercus glauca) stand measured by sap-flow method in a karst rocky terrain during dry season. Ecol Res 2009; 24:791–801 [View Article]
    [Google Scholar]
  16. Xk Q, Wang KL, Zhang CH. Effectiveness of ecological restoration projects in a karst region of southwest China assessed using vegetation succession mapping. Ecol Eng 2013; 54:245–253
    [Google Scholar]
  17. Yang Q, Jiang Z, Ma Z, Luo W, Xie Y et al. Relationship between karst rocky desertification and its distance to roadways in a typical karst area of Southwest China. Environ Earth Sci 2013; 70:295–302 [View Article]
    [Google Scholar]
  18. Xq W, Liu HM, Huang XL, Zhou T. Human driving forces: analysis of rocky desertification in karst region in Guanling County, Guizhou Province. Chinese Geographical Science 2011; 21:600–608
    [Google Scholar]
  19. Gams W, Hoekstra ES, Aptroot A. CBS course of Mycology, 4th ed. Baarn: Centraalbureau voor Schimmelcultures; 1998
    [Google Scholar]
  20. Samson RA, Stalpers JA, Verkerke W. A simplified technique to prepare fungal specimens for scanning electron microscopy. Cytobios 1979; 24:7–11[PubMed]
    [Google Scholar]
  21. Luo H, Liu Y, Fang L, Li X, Tang N et al. Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes. Appl Environ Microbiol 2007; 73:3916–3923 [View Article][PubMed]
    [Google Scholar]
  22. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications 1990; 18:315–322
    [Google Scholar]
  23. Koster B, Wong B, Straus N, Malloch D. A multi-gene phylogeny for Stachybotrys evidences lack of trichodiene synthase (tri5) gene for isolates of one of three intrageneric lineages. Mycol Res 2009; 113:877–886 [View Article][PubMed]
    [Google Scholar]
  24. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999; 99:553–556
    [Google Scholar]
  25. Groenewald JZ, Nakashima C, Nishikawa J, Shin HD, Park JH et al. Species concepts in Cercospora: spotting the weeds among the roses. Stud Mycol 2013; 75:115–170 [View Article][PubMed]
    [Google Scholar]
  26. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995; 61:1323–1330[PubMed]
    [Google Scholar]
  27. O'Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 1998; 95:2044–2049 [View Article][PubMed]
    [Google Scholar]
  28. Lombard L, Chen SF, Mou X, Zhou XD, Crous PW et al. New species, hyper-diversity and potential importance of Calonectria spp. from Eucalyptus in South China. Stud Mycol 2015; 80:151–188 [View Article][PubMed]
    [Google Scholar]
  29. Kindermann J, El-Ayouti Y, Samuels GJ, Kubicek CP. Phylogeny of the genus Trichoderma based on sequence analysis of the internal transcribed spacer region 1 of the rDNA cluster. Fungal Genet Biol 1998; 24:298–309 [View Article][PubMed]
    [Google Scholar]
  30. Lin CG et al. Stachybotrys-like taxa from karst areas and a checklist of stachybotrys-like species from Thailand. Mycosphere 2016; 7:1273–1291 [View Article]
    [Google Scholar]
  31. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
    [Google Scholar]
  32. Higgins DG. CLUSTAL V: multiple alignment of DNA and protein sequences. Methods Mol Biol 1994; 25:307–318 [View Article][PubMed]
    [Google Scholar]
  33. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 1999; 41:95–98
    [Google Scholar]
  34. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  35. Stamatakis A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 2006; 22:2688–2690 [View Article][PubMed]
    [Google Scholar]
  36. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  37. Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003; 19:1572–1574 [View Article][PubMed]
    [Google Scholar]
  38. Posada D. jModelTest: phylogenetic model averaging. Mol Biol Evol 2008; 25:1253–1256 [View Article][PubMed]
    [Google Scholar]
  39. Rambaut A. FigTree v1.4.2; 2012 Available from http://tree.bio.ed.ac.uk /software/figtree/
  40. Bisby GR, Ellis MB. Stachybotrys dichroa Grove. Trans Br Mycol Soc 1949; 32:158–161 [View Article]
    [Google Scholar]
  41. Koster B, Scott J, Wong B, Malloch D, Straus N. A geographically diverse set of isolates indicates two phylogenetic lineages within Stachybotrys chartarum . Can J Bot 2003; 81:633–643 [View Article]
    [Google Scholar]
  42. Haugland RA, Heckman JL. Identification of putative sequence specific PCR primers for detection of the toxigenic fungal species Stachybotrys chartarum . Mol Cell Probes 1998; 12:387–396 [View Article][PubMed]
    [Google Scholar]
  43. Haugland RA, Vesper SJ, Harmon SM. Phylogenetic relationships of Memnoniella and Stachybotrys species and evaluation of morphological features for Memnoniella species identification. Mycologia 2001; 93:54–65 [View Article]
    [Google Scholar]
  44. Jarvis BB. Chemistry and toxicology of molds isolated from water-damaged buildings. Adv Exp Med Biol 2002; 504:43–52 [View Article][PubMed]
    [Google Scholar]
  45. Hawksworth DL, Crous PW, Redhead SA, Reynolds DR, Samson RA et al. The Amsterdam declaration on fungal nomenclature. IMA Fungus 2011; 2:155–162
    [Google Scholar]
  46. Rossman AY, Seifert KA, Samuels GJ, Minnis AM, Schroers HJ et al. Genera in Bionectriaceae, Hypocreaceae, and Nectriaceae (Hypocreales) proposed for acceptance or rejection. IMA Fungus 2013; 4:41–51 [View Article][PubMed]
    [Google Scholar]
  47. Booth C. Studies of Pyrenomycetes. I. Four species of Chaetosphaeria, two with Catenularia conidia. II. Melanopsamma pomiformis and its Stachybotrys conidia . Mycological Papers 1957; 68:1–27
    [Google Scholar]
  48. Saccardo PA. Fungi Italici autographice delineati. Michelia 1878; 1:326–350
    [Google Scholar]
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