RT Journal Article SR Electronic(1) A1 Ramírez-Bahena, Martha Helena A1 Salazar, Sergio A1 Santín, Primitivo Julio A1 Sánchez-Rodríguez, Juan Antonio A1 Fernández-Pascual, Mercedes A1 Igual, José Mariano A1 Santa-Regina, Ignacio A1 Peix, ÁlvaroYR 2019 T1 Pseudomonas edaphica sp. nov., isolated from rhizospheric soil of Cistus ladanifer L. in Spain JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 10 SP 3141 OP 3147 DO https://doi.org/10.1099/ijsem.0.003603 PB Microbiology Society, SN 1466-5034, AB During a study on biodiversity of bacteria inhabiting rhizospheric soil of rockrose (Cistus ladanifer L.), we isolated a strain coded RD25T in a soil from Northern Spain. The 16S rRNA gene sequence showed 99.5 % identity with respect to the closest related species Pseudomonas brenneri DSM15294T, and 99.4 % with respect to P. paralactis WS4672T. The following related Pseudomonas species showed 99.3 % or less identity, and therefore RD25T was classified within genus Pseudomonas . The phylogenetic analysis of 16S rRNA and the housekeeping genes rpoB, rpoD and gyrB suggested that this strain could be a novel species. The strain RD25T has several polar-subpolar flagella. It can grow at 36 °C, at 0–6 % NaCl concentration and a range of pH 5–9. Positive for arginine dihydrolase and urease production, and negative for reduction of nitrate. The strain is catalase and oxidase positive. Major fatty acids are C16 : 1 ω7c / C16 : 1 ω6c in summed feature 3, C16 : 0, and C18 : 1 ω7c / C18 : 1 ω6c in summed feature 8. The respiratory ubiquinone is Q9. The DNA G+C content was 59.9 mol%. The digital DNA–DNA hybridisation average values (dDDH) ranged between 30–61.2 % relatedness and the ANIb values ranged between 93.9–80.5 % with respect to the type strains of the closely related species. Therefore, the genotypic, genomic, phenotypic and chemotaxonomic data support the classification of strain RD25 as a novel species of genus Pseudomonas , for which the name P. edaphica sp. nov. is proposed. The type strain is RD25T (=LMG 30152T=CECT 9373T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003603