1887

Abstract

During a study on biodiversity of bacteria inhabiting rhizospheric soil of rockrose ( L.), we isolated a strain coded RD25 in a soil from Northern Spain. The 16S rRNA gene sequence showed 99.5 % identity with respect to the closest related species DSM15294, and 99.4 % with respect to WS4672. The following related species showed 99.3 % or less identity, and therefore RD25 was classified within genus . The phylogenetic analysis of 16S rRNA and the housekeeping genes , and suggested that this strain could be a novel species. The strain RD25 has several polar-subpolar flagella. It can grow at 36 °C, at 0–6 % NaCl concentration and a range of pH 5–9. Positive for arginine dihydrolase and urease production, and negative for reduction of nitrate. The strain is catalase and oxidase positive. Major fatty acids are C ω7 / C ω6 in summed feature 3, C, and C ω7 / C ω6 in summed feature 8. The respiratory ubiquinone is Q9. The DNA G+C content was 59.9 mol%. The digital DNA–DNA hybridisation average values (dDDH) ranged between 30–61.2 % relatedness and the ANIb values ranged between 93.9–80.5 % with respect to the type strains of the closely related species. Therefore, the genotypic, genomic, phenotypic and chemotaxonomic data support the classification of strain RD25 as a novel species of genus , for which the name sp. nov. is proposed. The type strain is RD25 (=LMG 30152=CECT 9373).

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2019-10-01
2024-03-29
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