sp. nov., isolated from faeces of Free

Abstract

Two novel strains (HT111-2 and HT170-2) of the genus were isolated from faecal samples collected on the Qinghai-Tibet Plateau, PR China. The isolates were Gram-stain-positive, rod-shaped, non-spore-forming bacteria with irregular circular colonies. Phylogenetic analysis and comparison of the 16S rRNA gene sequences demonstrated that the two strains form a subcluster and are closest to JCM 6256 (97.3 %) and DSM 11664 (97.2 %). Phylogenetic analysis of two housekeeping genes ( and ) found that strains HT111-2 and HT170-2 had the same closest relatives as the 16S rRNA gene sequence analysis did. The G+C content of strains HT111-2 and HT170-2 were 38.8 mol%. The values of DNA–DNA hybridization with known species were lower than the threshold (70%). Average nucleotide identity values of strain HT111-2 with JCM 6256 and DSM 11664 were 77.84 % and 76.85 %, respectively. The major fatty acids of strains HT111-2 and HT170-2 were C, Cω and C. Results of phenotypic, chemotaxonomic and phylogenetic analyses suggest strains HT111-2 and HT170-2 represent a novel species of the genus , for which the name sp. nov. is proposed with HT111-2 (=CGMCC 1.13855=KCTC 15803) as the type strain.

Funding
This study was supported by the:
  • Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: Not Applicable
  • Jiangsu Agricultural Science and Technology Independent Innovation Fund (CN) (Award 2018ZX10305409-003)
    • Principle Award Recipient: Not Applicable
  • Key Technology Research and Development Program of Shandong (CN) (Award 2018YFC1200102)
    • Principle Award Recipient: Not Applicable
  • National Science and Technology Major Project of China (Award 2018ZX10712001-007)
    • Principle Award Recipient: Not Applicable
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003598
2019-09-27
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/1/11.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003598&mimeType=html&fmt=ahah

References

  1. Bohak I, Back W, Richter L, Ehrmann M, Ludwig W et al. Lactobacillus amylolyticus sp. nov., isolated from beer malt and beer wort. Syst Appl Microbiol 1998; 21:360364 [View Article]
    [Google Scholar]
  2. Hammes WP, Hertel C. Genus Lactobacillus Beijerink, 1901. In De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W. (editors) Bergey’s Manual of Systematic Bacteriology vol 3, 2nd edn. Berlin: Springer; 2009 pp 465–510
    [Google Scholar]
  3. Tanasupawat S, Shida O, Okada S, Komagata K. Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 2000; 50:14791485 [View Article]
    [Google Scholar]
  4. Roos S, Engstrand L, Jonsson H. Lactobacillus gastricus sp. nov., Lactobacillus antri sp. nov., Lactobacillus kalixensis sp. nov. and Lactobacillus ultunensis sp. nov., isolated from human stomach mucosa. Int J Syst Evol Microbiol 2005; 55:77–82 [View Article]
    [Google Scholar]
  5. Endo A, Irisawa T, Futagawa-Endo Y, Salminen S, Ohkuma M et al. Lactobacillus faecis sp. nov., isolated from animal faeces. Int J Syst Evol Microbiol 2013; 63:4502–4507 [View Article]
    [Google Scholar]
  6. Endo A, Futagawa-Endo Y, Sakamoto M, Kitahara M, Dicks LMT. Lactobacillus florum sp. nov., a fructophilic species isolated from flowers. Int J Syst Evol Microbiol 2010; 60:2478–2482 [View Article]
    [Google Scholar]
  7. De Bruyne K, Camu N, De Vuyst L, Vandamme P. Lactobacillus fabifermentans sp. nov. and Lactobacillus cacaonum sp. nov., isolated from Ghanaian cocoa fermentations. Int J Syst Evol Microbiol 2009; 59:7–12 [View Article]
    [Google Scholar]
  8. Denev SA, Suzuki I, Kimoto H. Role of lactobacilli in human and animal health: a review. animal scince journal 2000; 6:14
    [Google Scholar]
  9. Forssten SD, Lahtinen SJ, Ouwehand AC. The intestinal microbiota and probiotics: Probiotic . Bacteria and Enteric Infections 2011
    [Google Scholar]
  10. Gilliland SE. Acidophilus milk products: a review of potential benefits to consumers. J Dairy Sci 1989; 72:2483–2494 [View Article]
    [Google Scholar]
  11. Gilliland SE, Speck ML. Antagonistic action of Lactobacillus acidophilus toward intestinal and foodborne pathogens in associative cultures1 . J Food Prot 1977; 40:820–823 [View Article]
    [Google Scholar]
  12. Mital BK, Garg SK, Anticarcinogenic GSK. Anticarcinogenic, hypocholesterolemic, and antagonistic activities of Lactobacillus acidophilus . Crit Rev Microbiol 1995; 21:175–214 [View Article]
    [Google Scholar]
  13. Holzapfel WH, Haberer P, Geisen R, Björkroth J, Schillinger U. Taxonomy and important features of probiotic microorganisms in food and nutrition. Am J Clin Nutr 2001; 73:365S373 [View Article]
    [Google Scholar]
  14. Jin D, Chen C, Li L, Lu S, Li Z et al. Dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis . BMC Microbiol 2013; 13:141 [View Article]
    [Google Scholar]
  15. Wang C, Huang Y, Li L, Guo J, Wu Z et al. Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread. Int J Syst Evol Microbiol 2018; 68:703–708 [View Article]
    [Google Scholar]
  16. Taranto MP, Sesma F, Pesce de Ruiz Holgado A, de Valdez GF. Bile salts hydrolase plays a key role on cholesterol removal by Lactobacillus reuteri . Biotechnol Lett 1997; 19:845–847 [View Article]
    [Google Scholar]
  17. Horáčková Šárka, Plocková M, Demnerová K. Importance of microbial defence systems to bile salts and mechanisms of serum cholesterol reduction. Biotechnol Adv 2018; 36:682–690 [View Article]
    [Google Scholar]
  18. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:4849 [View Article]
    [Google Scholar]
  19. McFrederick QS, Vuong HQ, Rothman JA. Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers. Int J Syst Evol Microbiol 2018; 68:1879–1884 [View Article]
    [Google Scholar]
  20. Jung MY, Lee SH, Lee M, Song JH, Chang JY. Lactobacillus allii sp. nov. isolated from scallion kimchi. Int J Syst Evol Microbiol 2017; 67:4936–4942 [View Article]
    [Google Scholar]
  21. Chang C-huan, Chen Y-sheng, Lee T-tai, Chang Y-chung, Yu B. Lactobacillus formosensis sp. nov., a lactic acid bacterium isolated from fermented soybean meal. Int J Syst Evol Microbiol 2015; 65:101–106 [View Article]
    [Google Scholar]
  22. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003598
Loading
/content/journal/ijsem/10.1099/ijsem.0.003598
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed