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Volume 69, Issue 10

sp. nov., isolated from forest soil Free

Abstract

A novel bacterial strain, designated NEAU-SA2, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach. The cells were aerobic, Gram-stain-positive, non-flagellated and rod-coccus-shaped. The strain grew optimally at 28 °C, pH 7.0 and with 0 % NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus and was closely related to DSM 24664 (98.89 %) and CCTCC AB 2017271 (98.41 %), which was further confirmed by multilocus sequence analysis. The major cellular fatty acids were -C, -C and C; MK-9(H) was the predominant respiratory quinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The peptidoglycan type was A3α, and the cell-wall sugars were glucose and galactose. The genomic G+C content of strain NEAU-SA2 was 67.04 mol%. The average nucleotide identity values between NEAU-SA2 and DSM 24664 and CCTCC AB 2017271 were 88.57–90.94 %. The digital DNA–DNA hybridisation values between strain NEAU-SA2 and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa. Therefore, strain NEAU-SA2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-SA2 (=DSM 106718=CCTCC AB 2017272).

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Keyword(s): Arthrobacter celericrescens and MLSA
© 2019 IUMS
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2019-10-01
2024-04-16
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Arthrobacter celericrescens sp. nov., isolated from forest soil
Int J Syst Evol Microbiol 69, 3093 (2019); https://doi.org/10.1099/ijsem.0.003597
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