f.a., sp. nov., a new yeast species isolated from the gut of the rhinoceros beetle Free

Abstract

Four strains, NYNU 15610, NYNU 15612, NYNU 15613 and NYNU 15615, of a novel ascomycetous yeast were isolated from the gut of (Coleoptera: Scarabeidae) collected from two different localities in Henan Province, Central PR China. The four strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions. Sequence analyses revealed that this novel species represents a member of the genus . It differed from its closest known species , and , by 8.4–9.2 % sequence divergence (33–40 nt substitutions and 7–12 gaps over 509 bases) in the D1/D2 sequences. The formation of ascospores was not observed on various sporulation media. In contrast to , and , the novel yeast species was unable to assimilate succinate, ethanol, ethylamine, cadaverine and 10 % NaCl plus 5 % glucose, but was able to grow in vitamin-free medium. The name f.a., sp. nov. is proposed to accommodate these strains, with NYNU 15613 as the holotype.

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2019-10-01
2024-03-28
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