Four strains, NYNU 15610, NYNU 15612, NYNU 15613 and NYNU 15615, of a novel ascomycetous yeast were isolated from the gut of Allomyrina dichotoma (Coleoptera: Scarabeidae) collected from two different localities in Henan Province, Central PR China. The four strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions. Sequence analyses revealed that this novel species represents a member of the genus Metschnikowia. It differed from its closest known species Metschnikowia zobellii, Metschnikowiaaustralis and Metschnikowia bicuspidata, by 8.4–9.2 % sequence divergence (33–40 nt substitutions and 7–12 gaps over 509 bases) in the D1/D2 sequences. The formation of ascospores was not observed on various sporulation media. In contrast to M. zobellii, M. australis and M. bicuspidata, the novel yeast species was unable to assimilate succinate, ethanol, ethylamine, cadaverine and 10 % NaCl plus 5 % glucose, but was able to grow in vitamin-free medium. The name Metschnikowia baotianmanensis f.a., sp. nov. is proposed to accommodate these strains, with NYNU 15613 as the holotype.
LachanceMA.
Metschnikowia. In
KurtzmanCP,
FellJW,
BoekhoutT.
(editors) The Yeasts: A Taxonomic Study, 5th ed. vol. 2 Amsterdam, The Netherlands: Elsevier; 2011 pp. 575–620
KurtzmanCP,
RobnettCJ,
BasehoarE,
WardTJ.
Four new species of Metschnikowia and the transfer of seven Candida species to Metschnikowia and Clavispora as new combinations. Antonie van Leeuwenhoek2018; 111:2017–2035 [View Article][PubMed]
de VegaC,
AlbaladejoRG,
LachanceMA.
Metschnikowia maroccana f.a., sp. nov., a new yeast species associated with floral nectar from Morocco. Int J Syst Evol Microbiol2018; 68:2028–2035 [View Article][PubMed]
LachanceMA,
StarmerWT,
RosaCA,
BowlesJM,
BarkerJS et al. Biogeography of the yeasts of ephemeral flowers and their insects. FEMS Yeast Res2001; 1:1–8 [View Article][PubMed]
KuanCS,
IsmailR,
KwanZ,
YewSM,
YeoSK et al. Isolation and characterization of an atypical metschnikowia sp. strain from the skin scraping of a dermatitis patient. PLoS One2016; 11:e0156119 [View Article][PubMed]
de VegaC,
AlbaladejoRG,
GuzmánB,
SteenhuisenS–L,
JohnsonSD et al. Flowers as a reservoir of yeast diversity: description of Wickerhamiella nectarea f.a. sp. nov., and Wickerhamiella natalensis f.a. sp. nov. from South African flowers and pollinators, and transfer of related Candida species to the genus Wickerhamiella as new combinations. FEMS Yeast Res2017; 17:fox054 [View Article]
HuiFL,
NiuQH,
KeT,
LiuZ.
Candida ficus sp. nov., a novel yeast species from the gut of Apriona germari larvae. Int J Syst Evol Microbiol2012; 62:2805–2809 [View Article][PubMed]
HuiFL,
ChenL,
ChuXY,
NiuQH,
KeT.
Wickerhamomyces mori sp. nov., an anamorphic yeast species found in the guts of wood-boring insect larvae. Int J Syst Evol Microbiol2013; 63:1174–1178 [View Article][PubMed]
HuiFL,
ChenL,
LiZH,
NiuQH,
KeT.
Metschnikowia henanensis sp. nov., a new anamorphic yeast species isolated from rotten wood in China. Antonie van Leeuwenhoek2013; 103:899–904 [View Article][PubMed]
NguyenNH,
SuhSO,
BlackwellM.
Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects. Mycologia2007; 99:842–858 [View Article][PubMed]
KurtzmanCP,
FellJW,
BoekhoutT,
RobertV.
Methods for isolation, phenotypic characterization and maintenance of yeasts. In
KurtzmanCP,
FellJW,
BoekhoutT.
(editors) The Yeasts, A Taxonomic Study, 5th ed. vol. 1 Amsterdam: Elsevier; 2011 pp. 87–110
YarrowD.
Methods for the isolation, maintenance and identification of yeasts. In
KurtzmanCP,
FellJW.
(editors) The Yeasts, A Taxonomic Study, 4th ed. Amsterdam: Elsevier; 1998 pp. 77–100
WhiteTJ,
BrunsT,
LeeS,
TaylorJW.
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In
InnisMA,
GelfandDH,
SninskyJJ,
WhiteTJ.
(editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990 pp. 315–322
KurtzmanCP,
RobnettCJ.
Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek1998; 73:331–371 [View Article][PubMed]
AltschulSF,
MaddenTL,
SchäfferAA,
ZhangJ,
ZhangZ et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res1997; 25:3389–3402 [View Article][PubMed]
KimuraM.
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol1980; 16:111–120 [View Article][PubMed]
VuD,
GroenewaldM,
SzökeS,
CardinaliG,
EberhardtU et al. DNA barcoding analysis of more than 9000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol2016; 85:91–105 [View Article][PubMed]
KurtzmanCP,
RobnettCJ,
BasehoarE,
WardTJ.
Four new species of Metschnikowia and the transfer of seven Candida species to Metschnikowia and Clavispora as new combinations. Antonie van Leeuwenhoek2018; 111:2017–2035 [View Article][PubMed]
Mendonça-HaglerLC,
HaglerAN,
PhaffHJ,
TredickJ.
DNA relatedness among aquatic yeasts of the genus Metschnikowia and proposal of the species Metschnikowia australis comb. nov. Can J Microbiol1985; 31:905–909 [View Article][PubMed]
van UdenN,
Castelo-BrancoR.
Metschnikowiella zobellii sp.nov. and M. krissii sp.nov., two yeasts from the Pacific Ocean pathogenic for Daphnia magna. J Gen Microbiol1961; 26:141–148 [View Article][PubMed]
RenYC,
WangY,
ChenL,
KeT,
HuiFL et al.Wickerhamiella allomyrinae f.a., sp. nov., a yeast species isolated from the gut of the rhinoceros beetle Allomyrina dichotoma
. Int J Syst Evol Microbiol2014; 64:3856–3861 [View Article][PubMed]