1887

Abstract

Three novel actinobacterial strains, designated as TPS16, TPS81 and TPS83, were isolated from a sample of marine sediment collected from Tioman Island, Malaysia. The strains formed abundant branched substrate mycelia without fragmentation along with production of blue spores and blue diffusible pigment on soybean meal agar. The strains could grow at pH ranging from pH 6 to 12 and in 0–8 % (w/v) NaCl. Cell-wall hydrolysis showed the presence of -diaminopimelic acid. The strains were closely related to SCSIO 00652 (97.60 %) and YIM 690053 (96.87 %) based on phylogenetic analysis of 16S rRNA gene sequences. Multilocus sequence analysis including , and genes further confirmed that strain TPS16 represented a distinct branch within the family . The predominant menaquinones were MK-11(H), MK-10(H), MK-11(H) and MK-10(H), while the major fatty acids were found to be iso-C, anteiso-C, iso-C and Cω9. Genome sequencing revealed genome sizes of approximately 6 Mb and G+C contents of 73.8 mol%. A new genus, gen. nov., is proposed within the family based on polyphasic data and the type species is gen. nov., sp. nov. The type strain is TPS16 (=DSM 46825=TBRC 5138).

Keyword(s): Actinobacteria , genome and Malaysia
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2019-10-01
2019-10-20
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