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Volume 69, Issue 12

sp. nov., a bacterium isolated from cave soil Free

Abstract

A Gram-stain-negative, rod-shaped, non-motile, aerobic, catalase-negative and oxidase-positive bacterium, designated strain Sn-9-2, was isolated from a cave soil sample collected from Tiandong cave, Guizhou Province, south-west PR China. Growth occurred at 15–40 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 7.0–8.0) and with 0–1 % NaCl (w/v). The predominant respiration quinone was ubiquinone-10 (Q-10). The major cellular fatty acids were summed feature 8 (Cω7 or Cω6; 83.9 %) and C (5.8 %). The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, three unidentified phospholipids, two unidentified glycolipids, two unidentified polar lipids and one unidentified aminolipid. The DNA G+C content of strain Sn-9-2 was 67.5 mol%. Based on the results of 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain Sn-9-2 (MF958452) were identified as (FR733686) DSM 9035 (97.5 %), (jgi.1053054) DSM 432 (97.2 %) and ATCC 700314 RCTF01000015 (96.9 %). The average nucleotide identity values were 78.0, 77.4 and 77.6 % and the digital DNA–DNA hybridization values were 21.8, 22.0 and 18.8 % between strain Sn-9-2 and DSM 9035, DSM 432 and DSM 11105, respectively. The DNA–DNA hybridization data indicated that strain Sn-9-2 represented a novel genomic species. On the basis of the results of phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain Sn-9-2 should represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Sn-9-2 (=KCTC 62308=CCTCC AB 2018270).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Aquabacter cavernae sp. nov. , cave soil and polyphasic taxonomic
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2019-12-01
2024-04-25
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Aquabacter cavernae sp. nov., a bacterium isolated from cave soil
Int J Syst Evol Microbiol 69, 3716 (2019); https://doi.org/10.1099/ijsem.0.003585
/content/journal/ijsem/10.1099/ijsem.0.003585
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