1887

Abstract

A facultative halo-tolerant strain was isolated from olive brine waste, the effluent from the debittering process of table olives. Phenotypic and molecular characteristics showed clearly that the isolate represents a novel species. Based on the source of isolation, the new species has been named . It was found tolerant to high concentrations of NaCl (15 %) or sucrose (60 %) and it exhibits substantial growth under these conditions. Although the new species grew profusely at 37 °C, no growth was observed at 40 °C, conidia were avellaneous on all media. The description of the new species brings the total species of sect. to 15. The type strain of sp. nov. is NRRL 66783 (CCF 6208), its whole genome has been deposited as PRJNA498048.

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2019-09-01
2019-09-18
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References

  1. Klich MA. Biogeography of Aspergillus species in soil and litter. Mycologia 2002;94:21–27 [CrossRef][PubMed]
    [Google Scholar]
  2. Domsch KH, Gams W, Anderson TH. Compendium of soil fungi, 2nd ed. IHW-Verlag: Eching; 2007
    [Google Scholar]
  3. Stuart EA, Blank F. Aspergillosis of the ear; a report of twenty-nine cases. Can Med Assoc J 1955;72:334–337[PubMed]
    [Google Scholar]
  4. Roselle GA, Baird IM. Aspergillus flavipes group osteomyelitis. Arch Intern Med 1979;139:590–592 [CrossRef][PubMed]
    [Google Scholar]
  5. Barson WJ, Ruymann FB. Palmar aspergillosis in immunocompromised children. Pediatr Infect Dis 1986;5:264–268 [CrossRef][PubMed]
    [Google Scholar]
  6. Schultz RM, Johnson EG, Wisner ER, Brown NA, Byrne BA et al. Clinicopathologic and diagnostic imaging characteristics of systemic aspergillosis in 30 dogs. J Vet Intern Med 2008;22:851–859 [CrossRef][PubMed]
    [Google Scholar]
  7. Gehlot P, Purohit D, Singh S. Molecular diagnostics of human pathogenic Aspergillus species. Indian Journal of Biotechnology 2011;10:207–211
    [Google Scholar]
  8. Pore RS, Larsh HW. Aleuriospore formation in four related Aspergillus species. Mycologia 1967;59:318–325 [CrossRef]
    [Google Scholar]
  9. Tuomi T, Reijula K, Johnsson T, Hemminki K, Hintikka EL et al. Mycotoxins in crude building materials from water-damaged buildings. Appl Environ Microbiol 2000;66:1899–1904 [CrossRef][PubMed]
    [Google Scholar]
  10. Gorst-Allman CP, Steyn PS. Screening methods for the detection of thirteen common mycotoxins. J Chromatogr 1979;175:325–331 [CrossRef][PubMed]
    [Google Scholar]
  11. Greenhill AR, Blaney BJ, Shipton WA, Frisvad JC, Pue A et al. Mycotoxins and toxigenic fungi in sago starch from Papua New Guinea. Lett Appl Microbiol 2008;47:342–347 [CrossRef][PubMed]
    [Google Scholar]
  12. Valera HR, Gomes J, Lakshmi S, Gururaja R, Suryanarayan S et al. Lovastatin production by solid state fermentation using Aspergillus flavipes. Enzyme Microb Technol 2005;37:521–526 [CrossRef]
    [Google Scholar]
  13. Hurtado A, Reguant C, Bordons A, Rozès N. Lactic acid bacteria from fermented table olives. Food Microbiol 2012;31:1–8 [CrossRef][PubMed]
    [Google Scholar]
  14. Tresner HD, Hayes JA. Sodium chloride tolerance of terrestrial fungi. Appl Microbiol 1971;22:210–213[PubMed]
    [Google Scholar]
  15. Moustafa AF, Al-Musallam AA. Contribution to the fungal flora of Kuwait. Transactions of the British Mycological Society 1975;65:547–553 [CrossRef]
    [Google Scholar]
  16. Moustafa AF. Osmophilous fungi in the salt marshes of Kuwait. Can J Microbiol 1975;21:1573–1580 [CrossRef][PubMed]
    [Google Scholar]
  17. Butinar L, Frisvad JC, Gunde-Cimerman N. Hypersaline waters - a potential source of foodborne toxigenic aspergilli and penicillia. FEMS Microbiol Ecol 2011;77:186–199 [CrossRef][PubMed]
    [Google Scholar]
  18. Cantrell SA, Casillas-Martínez L, Molina M. Characterization of fungi from hypersaline environments of solar salterns using morphological and molecular techniques. Mycol Res 2006;110:962–970 [CrossRef][PubMed]
    [Google Scholar]
  19. Pawar V, Thirumalachar M. Studies on halophilic soil fungi from Bombay. Nova Hedwigia 1966;12:497–508
    [Google Scholar]
  20. Grishkan I, Nevo E, Wasser SP. Soil micromycete diversity in the hypersaline Dead Sea coastal area, Israel. Mycol Prog 2003;2:19–28 [CrossRef]
    [Google Scholar]
  21. Arzanlou M, Samadi R, Frisvad JC, Houbraken J, Ghosta Y. Two novel Aspergillus species from hypersaline soils of The National Park of Lake Urmia, Iran. Mycol Prog 2016;15:1081–1092 [CrossRef]
    [Google Scholar]
  22. Hubka V, Nováková A, Kolařík M, Jurjević Ž, Peterson SW. Revision of Aspergillus section Flavipedes: seven new species and proposal of section Jani sect. nov. Mycologia 2015;107:169–208 [CrossRef][PubMed]
    [Google Scholar]
  23. Visagie CM, Hirooka Y, Tanney JB, Whitfield E, Mwange K et al. Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world. Stud Mycol 2014;78:63–139 [CrossRef][PubMed]
    [Google Scholar]
  24. Siqueira JPZ. Clinical and environmental Aspergillus: morphological and molecular characterization, phylogeny, and antifungal susceptibility profile [dissertation]. Reus: Universitat Rovira i Virgili 2017
    [Google Scholar]
  25. Siqueira JPZ, Wiederhold N, Gené J, García D, Almeida MTG et al. Cryptic Aspergillus from clinical samples in the USA and description of a new species in section Flavipedes. Mycoses 2018;61:814–825 [CrossRef][PubMed]
    [Google Scholar]
  26. Crognale S, Pesciaroli L, Petruccioli M, D’Annibale A. Phenoloxidase-producing halotolerant fungi from olive brine wastewater. Process Biochem 2012;47:1433–1437 [CrossRef]
    [Google Scholar]
  27. Peterson SW, Jurjević Ž. New species of Talaromyces isolated from maize, indoor air, and other substrates. Mycologia 2017;109:537–556 [CrossRef][PubMed]
    [Google Scholar]
  28. Samson RA, Visagie CM, Houbraken J, Hong SB, Hubka V et al. Phylogeny, identification and nomenclature of the genus Aspergillus. Stud Mycol 2014;78:141–173 [CrossRef][PubMed]
    [Google Scholar]
  29. Ridgway R. Color Standards and Color Nomenclature Washington; 1912; Published by the author
    [Google Scholar]
  30. Peterson SW. Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. Mycologia 2008;100:205–226 [CrossRef][PubMed]
    [Google Scholar]
  31. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  32. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 2013;30:772–780 [CrossRef][PubMed]
    [Google Scholar]
  33. Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 2015;32:268–274 [CrossRef][PubMed]
    [Google Scholar]
  34. Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W et al. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 2010;59:307–321 [CrossRef][PubMed]
    [Google Scholar]
  35. Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh LS. UFBoot2: Improving the Ultrafast Bootstrap Approximation. Mol Biol Evol 2018;35:518–522 [CrossRef][PubMed]
    [Google Scholar]
  36. Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 1996;12:357–358[PubMed]
    [Google Scholar]
  37. Stanke M, Steinkamp R, Waack S, Morgenstern B. AUGUSTUS: a web server for gene finding in eukaryotes. Nucleic Acids Res 2004;32:W309–W312 [CrossRef][PubMed]
    [Google Scholar]
  38. Stanke M, Morgenstern B. AUGUSTUS: a web server for gene prediction in eukaryotes that allows user-defined constraints. Nucleic Acids Res 2005;33:W465–W467 [CrossRef][PubMed]
    [Google Scholar]
  39. Stanke M, Schöffmann O, Morgenstern B, Waack S. Gene prediction in eukaryotes with a generalized hidden Markov model that uses hints from external sources. BMC Bioinformatics 2006;7:62 [CrossRef][PubMed]
    [Google Scholar]
  40. Wang Y, Coleman-Derr D, Chen G, Gu YQ. OrthoVenn: a web server for genome wide comparison and annotation of orthologous clusters across multiple species. Nucleic Acids Res 2015;43:W78–W84 [CrossRef][PubMed]
    [Google Scholar]
  41. Sklenář F, Jurjević Ž, Zalar P, Frisvad JC, Visagie CM et al. Phylogeny of xerophilic aspergilli (subgenus Aspergillus) and taxonomic revision of section Restricti. Stud Mycol 2017;88:161–236 [CrossRef][PubMed]
    [Google Scholar]
  42. Jurjevic Z, Peterson SW, Horn BW. Aspergillus section Versicolores: nine new species and multilocus DNA sequence based phylogeny. IMA Fungus 2012;3:59–79 [CrossRef][PubMed]
    [Google Scholar]
  43. Houbraken J, Samson RA. Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Stud Mycol 2011;70:1–51 [CrossRef][PubMed]
    [Google Scholar]
  44. Kocsubé S, Perrone G, Magistà D, Houbraken J, Varga J et al. Aspergillus is monophyletic: evidence from multiple gene phylogenies and extrolites profiles. Stud Mycol 2016;85:199–213 [CrossRef][PubMed]
    [Google Scholar]
  45. Yilmaz N, Visagie CM, Houbraken J, Frisvad JC, Samson RA. Polyphasic taxonomy of the genus Talaromyces. Stud Mycol 2014;78:175–341 [CrossRef][PubMed]
    [Google Scholar]
  46. Houbraken J, Spierenburg H, Frisvad JC. Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species. Antonie van Leeuwenhoek 2012;101:403–421 [CrossRef][PubMed]
    [Google Scholar]
  47. Summerbell RC, Gueidan C, Schroers HJ, de Hoog GS, Starink M et al. Acremonium phylogenetic overview and revision of Gliomastix, Sarocladium, and Trichothecium. Stud Mycol 2011;68:139–162 [CrossRef][PubMed]
    [Google Scholar]
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