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Volume 69, Issue 9

Genomic metrics analyses indicate that is not a later synonym of Free

Abstract

and , both Gram-stain-positive and endospore-forming bacilli, have been considered to be a single species. However, a preliminary computation of their average nucleotide identity (ANI) values suggested that these species are not synonyms. Given this, the taxonomic attributions of these species were evaluated through genomic and phylogenomic approaches. Although the identity of 16S rRNA gene sequences of DSM 1735 and ATCC 35681 are above the circumscription species threshold, genomic metrics analyses indicate otherwise. ANI, gANI and OrthoANI values computed from their genome sequences were around 92 %, below the species limits. Digital DNA–DNA hybridization and MUMi estimations also corroborated these observations. In fact, in all metrics, JH29 seemed to be more similar to ATCC 35681 than DSM 1735. Phylogenetic analyses based on concatenated core-proteome and concatenated , , and genes confirmed that is the closest species to A review of the phenotypic profiles from these three species revealed that their biochemical repertoires are very similar, although ATCC 35681 can be differentiated from DSM1735 in 13 among more than 90 phenotypic traits. Considering phylogenetic and genomic analyses, should be considered as an independent species, and not as a later synonym of .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ANI , genome metrics , Paenibacillus azotofixans , Paenibacillus durus , Paenibacillus zanthoxyli and reclassification
© 2019 The Authors
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2019-09-01
2024-04-25
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Genomic metrics analyses indicate that Paenibacillus azotofixans is not a later synonym of Paenibacillus durus
Int J Syst Evol Microbiol 69, 2870 (2019); https://doi.org/10.1099/ijsem.0.003572
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