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Abstract

Strain HaS4 is an aerobic sulfur-oxidizing bacterium isolated from water of Lake Harutori in Japan. It was isolated and partially characterized in a previous study, but its taxonomic status has not been determined. The previous study revealed that the strain is an obligate chemolithoautotroph which grows at temperatures ranging from 0 to 25 °C (optimum, 22 °C) and pH from pH 6.2 to 8.8 (optimum, pH 6.6–7.4). In this study, further characterization of the strain was made to describe it as representative of a novel species. Cells of strain HaS4 are rod-shaped, 1.6–2.5 µm long, 0.7–0.9 µm wide and Gram-stain-negative. Major cellular fatty acids were summed feature 3 (Cω7 and/or Cω6), summed feature 8 (Cω7 and/or Cω6) and C. Phylogenetic analysis based on the 16S rRNA gene indicated that the strain is related to the genus , but phylogenetically distinct from the type strains of existing species in the genus. On the basis of its phylogenetic and phenotypic properties, strain HaS4 (=NBRC 112315=BCRC 81110) is proposed as type strain of a new non-marine species of the genus with the name sp. nov.

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/content/journal/ijsem/10.1099/ijsem.0.003567
2019-09-01
2019-09-22
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