@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003563, author = "Fu, Mei Ling and Gu, Chun Tao", title = "Lactobacillus huachuanensis sp. nov., isolated from Chinese traditional pickle", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "9", pages = "2807-2814", doi = "https://doi.org/10.1099/ijsem.0.003563", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003563", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "pickle", keywords = "Lactobacillus", keywords = "novel species", keywords = "lactic acid bacteria", abstract = "A Gram-stain-positive bacterial strain, 395-6.2T, was isolated from traditional pickle in Heilongjiang Province, PR China. The bacterium was characterised by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, average nucleotide identity (ANI) analysis, in silico DNA–DNA hybridisation (isDDH) and an analysis of phenotypic features. Analysis of the 16S rRNA gene sequence showed that strain 395-6.2T was phylogenetically related to Lactobacillus farciminis , Lactobacillus formosensis , Lactobacillus futsaii , Lactobacillus crustorum , Lactobacillus nuruki , Lactobacillus heilongjiangensis , Lactobacillus musae , Lactobacillus nantensis , Lactobacillus mindensis , Lactobacillus kimchiensis , Lactobacillus zhachilii, Lactobacillus alimentarius , Lactobacillus bobalius , Lactobacillus kimchii and Lactobacillus paralimentarius . Strain 395-6.2T exhibited 95.7–99.4 % 16S rRNA gene sequence similarities, 85.0–94.0 % pheS gene sequence similarities, 94.2–98.0 % rpoA gene sequence similarities to type strains of phylogenetically related species. ANI and isDDH values between strain 395-6.2T and type strains of phylogenetically related species were 77.9–87.1 % and 22.5–33.5 %, respectively. Based upon the data obtained in the present study, a novel species, Lactobacillus huachuanensis sp. nov., is proposed and the type strain is 395-6.2T (=CCM 8927T=NCIMB 15188T=LMG 31179T).", }