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Volume 69, Issue 10

Two novel species of the family : sp. nov. and sp. nov., from deep-sea sediment samples of Yap Trench Free

Abstract

Two novel strains, designated YLB-02 and YLB-04, were isolated from the deep-sea sediments of Yap Trench located in the Pacific Ocean. Cells of the strains were Gram-stain-positive, oxidase- and catalase-positive and rod-shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YLB-02 belonged to the genus and strain YLB-04 belonged to the genus . Strain YLB-02 showed similarities of 96.9 % with CCUG 53201, 96.3 % with CL-MP28, 96.1 % with J8B and 95.7 % with Ma-21. Strain YLB-04 showed the highest sequence similarity of 97.4 % with SYP-B691. The average nucleotide identity (ANI) and the DNA–DNA hybridisation (DDH) estimate values for strain YLB-02 and YLB-04 with their related type strains were below the respective threshold for species differentiation. The G+C contents of strains YLB-02 and YLB-04 were 37.3 and 45.4 mol%. The predominant (>10 %) cellular fatty acids of strain YLB-02 were iso-C, iso-C, iso-C and Cω7 alcohol, and those of strain YLB-04 were C, iso-C, anteiso-C and C. Their predominant ubiquinone was MK-7. The cell-wall peptidoglycan of strain YLB-02 contained glutamic acid, alanine, aspartic acid, lysine and ornithine, but no -diaminopimelic acid, while strain YLB-04 contained -diaminopimelic acid, glutamic acid, alanine, aspartic acid, lysine and ornithine. In addition to diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), the polar lipids of strain YLB-02 also consisted of an unidentified glycolipid (GL), two unidentified polar lipids (L1 and L2) and two unidentified phospholipids (PL1 and PL2), and those of strain YLB-04 also consisted of phosphatidylethanolamine (PE) and an unidentified phospholipid (PL). Based on phenotypic, genotypic and chemotaxonomic characteristics, two novel species are proposed, sp. nov. with YLB-02 (=MCCC 1A12699=JCM 32870) and sp. nov. with YLB-04 (=MCCC 1A12711=JCM 32872) as the type strains.

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Keyword(s): Bacillus piezotolerans sp. nov. , deep-sea sediment , Oceanobacillus piezotolerans sp. nov. , piezotolerans , Taxonomy and Yap Trench
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2019-10-01
2024-04-25
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Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp. nov. and Bacillus piezotolerans sp. nov., from deep-sea sediment samples of Yap Trench
Int J Syst Evol Microbiol 69, 3022 (2019); https://doi.org/10.1099/ijsem.0.003559
/content/journal/ijsem/10.1099/ijsem.0.003559
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