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Volume 69, Issue 9

sp. nov., isolated from an intestine of Nile tilapia () Free

Abstract

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, MRS2, was isolated from an intestine of Nile tilapia () collected from the Republic of Korea. Strain MRS2 grew optimally at 30 °C and in the presence of 0–2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain MRS2 clustered with the type strains of species. It exhibited the highest 16S rRNA gene sequence similarity (98.5 %) to the type strain of and sequence similarities of 97.4–97.6 % to the type strains of two other species. Strain MRS2 contained MK-6 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), iso-C 3-OH and iso-C as the major fatty acids. The major polar lipids of strain MRS2 were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C contents of strain MRS2 were 32.2 mol% or 30.65 mol%. Strain MRS2 exhibited DNA–DNA relatedness values of 12–20 % to the type strains of , and . The average nucleotide identity values between strain MRS2 and five strains of and were 84.8–91.0 %. The phylogenetic, genetic and differential phenotypic properties indicated that strain MRS2 is separated from species. On the basis of the data presented here, strain MRS2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MRS2 (=KCTC 62904=NBRC 113550).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Empedobacter tilapiae , Nile tilapia , novel species , Oreochromis niloticus and polyphasic taxonomy
© 2019 The Authors
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2019-09-01
2024-04-20
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Empedobacter tilapiae sp. nov., isolated from an intestine of Nile tilapia (Oreochromis niloticus)
Int J Syst Evol Microbiol 69, 2781 (2019); https://doi.org/10.1099/ijsem.0.003558
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