sp. nov., isolated from forest soil Free

Abstract

During a study of the Kyonggi University soil bacterial diversity, an aerobic, non-motile, Gram-stain-negative, non-spore-forming, rod-shaped, yellow pigmented bacterium, designated strain RD-2-33 was isolated. Strain RD-2-33 grew optimally at 28–35 °C and pH 7.0–7.5; hydrolysed gelatin and DNA; and tolerated 1 % of NaCl. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RD-2-33 clustered with the genus . The closest member was ARSA-19 (97.1 % sequence similarity) and ARSA-15 (96.7 %). Sole respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine and an unidentified polar lipid. The major cellular fatty acids were iso-C, iso-C 3-OH, and iso-C G. The DNA G+C content was 38.6 mol%. The average nucleotide identity (ANI) and DNA–DNA hybridisation relatedness between strain RD-2-33 and DSM 25687 were 75.2 and 19.3 %, respectively. Based on the polyphasic and phylogenetic data, strain RD-2-33 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RD-2-33 (=KEMB 9005–742=KACC 21178=NBRC 113789).

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2019-09-01
2024-03-29
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