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Volume 69, Issue 8

sp. nov., isolated from cow rumen fibrous content Free

Abstract

A novel propionate producing bacterium, strain JV5, was isolated from the rumen fibrous content of a Holstein Friesian dairy cow. Cells of strain JV5 were Gram-stain-positive, non-motile and aerotolerant. Growth occurred between 35 and 45 °C, with an optimum at 39 °C. The pH range for growth was 6.5–8, with an optimum at pH 7. The 16S rRNA gene sequence of strain JV5 was 98.4 and 96.5 % identical to those of DSM 15818 and DSM 21887, respectively. Genome wide average nucleotide identity and digital DNA–DNA hybridization values were 88.3 and 35.5 %, respectively, against DSM 15818. The G+C content of strain JV5 was 68.9  mol%. Strain JV5 did not produce urease and was able to metabolize glutamate, but not aspartate and glycine. Strain JV5 was able to ferment a range of substrates including certain simple and complex carbohydrates, sugar alcohols and amino acids. Chemotaxonomic analysis of strain JV5 revealed the presence of diamino pimelic acid isomers similar those found in , but different from The observed major (>10 %) cellular fatty acids in strain JV5 (C ω9, anteiso-C, C, C and C alcohol) were also different from those observed in and . Based on these findings, a novel species is proposed within the genus , sp. nov. (type strain JV5=DSM 106771=TISTR 2629).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , dairy cow , methylcellulose , Propionibacterium and rumen fibres
© 2019 IUMS
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2019-08-01
2024-04-20
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Propionibacterium ruminifibrarum sp. nov., isolated from cow rumen fibrous content
Int J Syst Evol Microbiol 69, 2584 (2019); https://doi.org/10.1099/ijsem.0.003544
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