@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003533, author = "Barbeyron, Tristan and Zonta, Erwann and Le Panse, Sophie and Duchaud, Eric and Michel, Gurvan", title = "Alteromonas fortis sp. nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "8", pages = "2514-2521", doi = "https://doi.org/10.1099/ijsem.0.003533", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003533", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "iota-carrageenase", keywords = "Alteromonadaceae", keywords = "Alteromonas fortis", abstract = "Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method. Cells were Gram-stain-negative, strictly aerobic, non-flagellated, ovoid or rod-shaped and grew optimally at 20–25 °C, at pH 6–9 and with 2–4 % NaCl. Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources. The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0. The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid. Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae , class Gammaproteobacteria ). Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2–98.6 % to other species of the genus Alteromonas . The DNA G+C content of strain 1T was determined to be 43.9 mol%. Digital DNA–DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively. The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas , for which the name Alteromonas fortis sp. nov. is proposed. The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).", }