An anaerobic, Gram-stain-positive, spore-forming bacterium, designated strain PYR-10T, was isolated from a mesophilic methanogenic consortium. Cells were 0.7–1.2×6.0–6.3 µm, straight or slightly curved rods, with flagellar motility. Growth was observed in PYG (peptone-yeast glucose) medium at pH 5.5–8.0 (optimum, pH 6.5), 30–55 °C (45 °C) and in NaCl concentrations of 0–15 g l−1 (0 g l−1). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PYR-10T belongs to the genus Clostridium. The strain showed 95.4, 93.7, 93.5 and 93.0 % 16S rRNA gene sequence similarity to Clostridium swellfunianum DSM 27788T, Clostridium pascui DSM 10365T, Clostridium pasteurianum DSM 525T and Clostridium punense DSM 28650T, respectively. The genomic DNA G+C content was 27.7 mol%. The major cellular fatty acids of strain PYR-10T were iso-C15 : 0, C16 : 0, C16 : 0 DMA, anteiso-C15 : 0 and C14 : 0. The main polar lipids were glycolipid, phosphoaminoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylethanolamine and lipids. An unknown menaquinone was detected. 2,6-Diaminopimelic acid was not detected. The whole-cell sugars contained ribose and lower amounts of glucose. Based on the results of phylogenetic, chemotaxonomic and phenotypic analyses, strain PYR-10T represents a novel species of the genus Clostridium, for which the name Clostridium prolinivorans sp. nov. is proposed. The type strain is strain PYR-10T (=JCM 33161T=CCAM 531T=CGMCC 1.5286T).
Agricultural Science and Technology Innovation Program (ASTIP), the Chinese Academy of Agricultural Sciences and the Infrastructure
(Award CAAS-ASTIP-2016-BIOMA)
Facility Development Program of Sichuan Province
(Award 2018TJPT0004)
Science and Technology Program of Sichuan Province, China
(Award 2017JY0242)
Fundamental Research Funds for Central Non-profit Scientific Institution, China
(Award 1610012016023)
SchnurerA,
SchinkB,
SvenssonBH.
Clostridium ultunense sp. nov., a mesophilic bacterium oxidizing acetate in syntrophic association with a hydrogenotrophic methanogenic bacterium. Int J Syst Bacteriol1996; 46:1145–1152 [View Article][PubMed]
WesterholmM,
LevénL,
SchnürerA.
Bioaugmentation of syntrophic acetate-oxidizing culture in biogas reactors exposed to increasing levels of ammonia. Appl Environ Microbiol2012; 78:7619–7625 [View Article][PubMed]
TindallBJ,
Rosselló-MóraR,
BusseHJ,
LudwigW,
KämpferP.
Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol2010; 60:249–266 [View Article][PubMed]
ZhangX,
TuB,
DaiLR,
LawsonPA,
ZhengZZ et al.Petroclostridium xylanilyticum gen. nov., sp. nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam. nov. Int J Syst Evol Microbiol2018; 68:3197–3211 [View Article][PubMed]
LiuC,
HuangD,
LiuL,
ZhangJ,
DengY et al.Clostridium swellfunianum sp. nov., a novel anaerobic bacterium isolated from the pit mud of Chinese Luzhou-flavor liquor production. Antonie van Leeuwenhoek2014; 106:817–825 [View Article][PubMed]
KämpferP,
KroppenstedtRM.
Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol1996; 42:989–1005 [View Article]
TindallBJ,
SikorskiJ,
SmibertRA,
KriegNR.
Phenotypic characterization and the principles of comparative systematics. In
ReddyCA,
BeveridgeTJ,
BreznakJA,
MarzlufG,
SchmidtTM et al.
(editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC, USA: Snyder ASM Press; 2007 pp. 330–393
TindallBJ.
A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol1990; 13:128–130 [View Article]
StaneckJL,
RobertsGD.
Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol1974; 28:226–231[PubMed]
RhulandLE,
WorkE,
DenmanRF,
HoareDS.
The behavior of the isomers of α,ε-diaminopimelic acid on paper Chromatograms. J Am Chem Soc1955; 77:4844–4846 [View Article]
WildeE,
CollinsMD,
HippeH.
Clostridium pascui sp. nov., a new glutamate-fermenting sporeformer from a pasture in Pakistan. Int J Syst Bacteriol1997; 47:164–170 [View Article][PubMed]