sp. nov., a thermophilic bacterium isolated from an anaerobic reactor degrading propionate Free

Abstract

An anaerobic, Gram-stain-positive, spore-forming bacterium, designated strain PYR-10, was isolated from a mesophilic methanogenic consortium. Cells were 0.7–1.2×6.0–6.3 µm, straight or slightly curved rods, with flagellar motility. Growth was observed in PYG (peptone-yeast glucose) medium at pH 5.5–8.0 (optimum, pH 6.5), 30–55 °C (45 °C) and in NaCl concentrations of 0–15 g l (0 g l). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PYR-10 belongs to the genus . The strain showed 95.4, 93.7, 93.5 and 93.0 % 16S rRNA gene sequence similarity to DSM 27788, DSM 10365, DSM 525 and DSM 28650, respectively. The genomic DNA G+C content was 27.7 mol%. The major cellular fatty acids of strain PYR-10 were iso-C, C, C DMA, anteiso-C and C. The main polar lipids were glycolipid, phosphoaminoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylethanolamine and lipids. An unknown menaquinone was detected. 2,6-Diaminopimelic acid was not detected. The whole-cell sugars contained ribose and lower amounts of glucose. Based on the results of phylogenetic, chemotaxonomic and phenotypic analyses, strain PYR-10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain PYR-10 (=JCM 33161=CCAM 531=CGMCC 1.5286).

Funding
This study was supported by the:
  • Agricultural Science and Technology Innovation Program (ASTIP), the Chinese Academy of Agricultural Sciences and the Infrastructure (Award CAAS-ASTIP-2016-BIOMA)
  • Facility Development Program of Sichuan Province (Award 2018TJPT0004)
  • Science and Technology Program of Sichuan Province, China (Award 2017JY0242)
  • Fundamental Research Funds for Central Non-profit Scientific Institution, China (Award 1610012016023)
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2019-06-14
2024-03-28
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References

  1. Lawson PA. The taxonomy of the genus Clostridium: current status and future perspectives. Microbiology China 2016; 43:1070–1074
    [Google Scholar]
  2. Rainey FA, Holley BJ, Small A, Genus I. Clostridium Prazmowski 1880, 23. Bergeys Manual of Systematic Bacteriology New York: Springer; 2009
    [Google Scholar]
  3. Schnurer A, Schink B, Svensson BH. Clostridium ultunense sp. nov., a mesophilic bacterium oxidizing acetate in syntrophic association with a hydrogenotrophic methanogenic bacterium. Int J Syst Bacteriol 1996; 46:1145–1152 [View Article][PubMed]
    [Google Scholar]
  4. Westerholm M, Levén L, Schnürer A. Bioaugmentation of syntrophic acetate-oxidizing culture in biogas reactors exposed to increasing levels of ammonia. Appl Environ Microbiol 2012; 78:7619–7625 [View Article][PubMed]
    [Google Scholar]
  5. Touzel JP, Albagnac G. Isolation and characterization of Methanococcus mazei strain MC 3 . FEMS Microbiol Lett 1983; 16:241–245 [View Article]
    [Google Scholar]
  6. Hungate R. A roll tube method for cultivation of strict anaerobes. Methods in microbiology 1969; 3B:117–132
    [Google Scholar]
  7. Hungate RE, Macy J. The roll-tube method for cultivation of strict anaerobes. Bull Ecol Res Comm 1973; 17:123–126
    [Google Scholar]
  8. Macy JM, Snellen JE, Hungate RE. Use of syringe methods for anaerobiosis. Am J Clin Nutr 1972; 25:1318–1323 [View Article][PubMed]
    [Google Scholar]
  9. Huang Y, Sun Y, Ma S, Chen L, Zhang H et al. Isolation and characterization of Keratinibaculum paraultunense gen. nov., sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity. FEMS Microbiol Lett 2013; 345:56–63 [View Article][PubMed]
    [Google Scholar]
  10. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  11. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  13. Tindall BJ, Rosselló-Móra R, Busse HJ, Ludwig W, Kämpfer P. Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 2010; 60:249–266 [View Article][PubMed]
    [Google Scholar]
  14. Wang C, Huang Y, Li L, Guo J, Wu Z et al. Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread. Int J Syst Evol Microbiol 2018; 68:703–708 [View Article][PubMed]
    [Google Scholar]
  15. Zhang X, Tu B, Dai LR, Lawson PA, Zheng ZZ et al. Petroclostridium xylanilyticum gen. nov., sp. nov., a xylan-degrading bacterium isolated from an oilfield, and reclassification of clostridial cluster III members into four novel genera in a new Hungateiclostridiaceae fam. nov. Int J Syst Evol Microbiol 2018; 68:3197–3211 [View Article][PubMed]
    [Google Scholar]
  16. Liu C, Huang D, Liu L, Zhang J, Deng Y et al. Clostridium swellfunianum sp. nov., a novel anaerobic bacterium isolated from the pit mud of Chinese Luzhou-flavor liquor production. Antonie van Leeuwenhoek 2014; 106:817–825 [View Article][PubMed]
    [Google Scholar]
  17. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996; 42:989–1005 [View Article]
    [Google Scholar]
  18. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC, USA: Snyder ASM Press; 2007 pp. 330–393
    [Google Scholar]
  19. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
    [Google Scholar]
  20. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
    [Google Scholar]
  21. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974; 28:226–231[PubMed]
    [Google Scholar]
  22. Rhuland LE, Work E, Denman RF, Hoare DS. The behavior of the isomers of α,ε-diaminopimelic acid on paper Chromatograms. J Am Chem Soc 1955; 77:4844–4846 [View Article]
    [Google Scholar]
  23. Wilde E, Collins MD, Hippe H. Clostridium pascui sp. nov., a new glutamate-fermenting sporeformer from a pasture in Pakistan. Int J Syst Bacteriol 1997; 47:164–170 [View Article][PubMed]
    [Google Scholar]
  24. Lanjekar VB, Marathe NP, Shouche YS, Ranade DR. Clostridium punense sp. nov., an obligate anaerobe isolated from healthy human faeces. Int J Syst Evol Microbiol 2015; 65:4749–4756 [View Article][PubMed]
    [Google Scholar]
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