RT Journal Article SR Electronic(1) A1 He, Weihong A1 Guo, Jing A1 Guo, Heng A1 An, Mingli A1 Huang, Wen A1 Wang, Yanan A1 Cai, HaiyuanYR 2019 T1 Sphingobacterium puteale sp. nov., isolated from a deep subsurface aquifer JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 11 SP 3356 OP 3361 DO https://doi.org/10.1099/ijsem.0.003521 PB Microbiology Society, SN 1466-5034, AB A bacterial strain M05W1-28T was isolated from a well that collected water for irrigation from a deep aquifer at a depth of 400 m. Cells were observed to be rod-shaped, non-motile, aerobic, stained Gram-negative. Optimal growth was obtained at pH 7.0 (range: 6.0–9.0), 28 °C (range: 15–37 °C) and 0 % NaCl (range: 0–1.5 %, w/v) in modified tryptic soy broth (mTSB) without added NaCl and R2A. The cells were found to be positive for catalase and oxidase activities. The major fatty acids (>10 %) were identified as summed feature 3 (C16 : 1  ω7c / C16 : 1  ω6c) and iso-C15 : 0. The major polar lipids were phosphatidylethanolamine, glycolipid, phosphoglycolipids, phospholipids, and unidentified lipids. The major respiratory quinone was menaquinone-7 (MK-7). The genomic G+C content of strain M05W1-28T was 40.7 %. Based on similarities of 16S rRNA gene sequences, strain M05W1-28T was affiliated with the genus Sphingobacterium , exhibiting the highest sequence similarities with S. multivorum LMG 8342T (97.5 %), S. ginsenosidimutans THG07T (97.1 %) and less than 97.0 % to other members of the genus. The average nucleotide identity (ANI) and digital DNA–DNA hybridisation values (dDDH) between M05W1-28T and S. multivorum LMG 8342T were 78.1 and 22.5 %, respectively. Phenotypic characteristics including enzyme activities and carbon source utilisation differentiated the strain from other Sphingobacterium species. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain M05W1-28T represented a novel species within the genus Sphingobacterium , for which the name Sphingobacterium puteale sp. nov. is proposed. The type strain is M05W1-28T (=CGMCC 1.13711T=KCTC 72027T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003521