1887

International Journal of Systematic and Evolutionary Microbiology

Microbacterium bovistercoris sp. nov., a novel actinomycete isolated from cow dung No Access

Abstract

A Gram-stain-positive, aerobic actinomycete strain, designated NEAU-LLE, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, north-east China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLE belonged to the genus Microbacterium , with highest sequence similarity to Microbacterium pseudoresistens DSM 22185 (98.2 %). The predominant menaquinones were MK-13 and MK-14. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid. The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. However, DNA–DNA relatedness and physiological and biochemical data showed that strain NEAU-LLE could be distinguished from its closest relative. Therefore, strain NEAU-LLE represents a novel species of the genus Microbacterium , for which the name Microbacteriumbovistercoris sp. nov is proposed, with NEAU-LLE (=CCTCC AA 2018025=JCM 32662) as the type strain.

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Keyword(s): 16S rRNA gene , Microbacterium bovistercoris sp. nov. and polyphasic analysis
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2019-06-18
2019-08-22

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References

  1. Orla-Jensen S. The Lactic Acid Bacteria Copenhagen: Høst and Son; 1919
    [Google Scholar]
  2. Collins MD, Jones D, Kroppenstedt RM. Reclassification of Brevibacterium imperiale (Steinhaus) and "Corynebacterium laevaniformans" (Dias and Bhat) in a Redefined Genus Microbacterium (Orla-Jensen), as Microbacterium imperiale comb. nov. and Microbacterium laevaniformans nom. rev.; comb. nov. Syst Appl Microbiol 1983;4: 65– 78 [CrossRef] [PubMed]
    [Google Scholar]
  3. Takeuchi M, Hatano K. Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int J Syst Bacteriol 1998;48 Pt 3: 739– 747 [CrossRef] [PubMed]
    [Google Scholar]
  4. Krishnamurthi S, Bhattacharya A, Schumann P, Dastager SG, Tang SK et al. Microbacterium immunditiarum sp. nov., an actinobacterium isolated from landfill surface soil, and emended description of the genus Microbacterium. Int J Syst Evol Microbiol 2012;62: 2187– 2193 [CrossRef] [PubMed]
    [Google Scholar]
  5. Fidalgo C, Riesco R, Henriques I, Trujillo ME, Alves A. Microbacterium diaminobutyricum sp. nov., isolated from Halimione portulacoides, which contains diaminobutyric acid in its cell wall, and emended description of the genus Microbacterium. Int J Syst Evol Microbiol 2016;66: 4492– 4500 [CrossRef] [PubMed]
    [Google Scholar]
  6. Piao C, Zheng W, Li Y, Liu C, Jin L et al. Two new species of the genus Streptomyces: Streptomyces camponoti sp. nov. and Streptomyces cuticulae sp. nov. isolated from the cuticle of Camponotus japonicus Mayr. Arch Microbiol 2017;199: 963– 970 [CrossRef] [PubMed]
    [Google Scholar]
  7. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16: 313– 340 [CrossRef]
    [Google Scholar]
  8. Kelly KL. Inter-society color council-national bureau of standards color-name charts illustrated with centroid colors published in US; 1964.
  9. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology American Society for Microbiology; 1994; pp. 607– 654
    [Google Scholar]
  10. Gordon RE, Barnett DA, Handerhan JE, Pang CH-N. Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 1974;24: 54– 63 [CrossRef]
    [Google Scholar]
  11. Yokota A, Tamura T, Hasegawa T, Huang LH. Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. Int J Syst Bacteriol 1993;43: 805– 812 [CrossRef]
    [Google Scholar]
  12. Jia F, Liu C, Wang X, Zhao J, Liu Q, Zhang J et al. Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae. Antonie van Leeuwenhoek 2013;103: 399– 408 [CrossRef] [PubMed]
    [Google Scholar]
  13. Xie QY, Lin HP, Li L, Brown R, Goodfellow M et al. Verrucosispora wenchangensis sp. nov., isolated from mangrove soil. Antonie van Leeuwenhoek 2012;102: 1– 7 [CrossRef] [PubMed]
    [Google Scholar]
  14. Ruan Z, Wang Y, Song J, Jiang S, Wang H et al. Kurthia huakuii sp. nov., isolated from biogas slurry, and emended description of the genus Kurthia. Int J Syst Evol Microbiol 2014;64: 518– 521 [CrossRef] [PubMed]
    [Google Scholar]
  15. Nakai R, Baba T, Niki H, Nishijima M, Naganuma T. Aurantimicrobium minutum gen. nov., sp. nov., a novel ultramicrobacterium of the family Microbacteriaceae, isolated from river water. Int J Syst Evol Microbiol 2015;65: 4072– 4079 [CrossRef] [PubMed]
    [Google Scholar]
  16. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983;29: 319– 322 [CrossRef]
    [Google Scholar]
  17. Lechevalier MP, Lechevalier HA. The chemotaxonomy of actinomycetes. In Dietz A, Thayer DW. (editors) Actinomycete Taxonomy Special Publication, vol 6. Society of Industrial Microbiology 1980; pp. 227– 291
    [Google Scholar]
  18. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2: 233– 241 [CrossRef]
    [Google Scholar]
  19. Collins MD. Chemical methods in bacterial systematics. In Goodfellow M, Minnikin DE. (editors) Isoprenoid Quinone Analyses in Bacterial Classification and Identification Academic Press; 1985; pp. 267– 284
    [Google Scholar]
  20. Wu C, Lu X, Qin M, Wang Y, Ruan J. Analysis of menaquinone compound in microbial cells by HPLC. Microbiology 1989;16: 176– 178
    [Google Scholar]
  21. Gao R, Liu C, Zhao J, Jia F, Yu C et al. Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora. Antonie van Leeuwenhoek 2014;105: 307– 315 [CrossRef] [PubMed]
    [Google Scholar]
  22. Xiang W, Liu C, Wang X, du J, Xi L, Lj X et al. Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol 2011;61: 1165– 1169 [CrossRef] [PubMed]
    [Google Scholar]
  23. Kim SB, Brown R, Oldfield C, Gilbert SC, Iliarionov S et al. Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 2000;50 Pt 6: 2031– 2036 [CrossRef] [PubMed]
    [Google Scholar]
  24. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  25. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4: 406– 425 [CrossRef] [PubMed]
    [Google Scholar]
  26. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33: 1870– 1874 [CrossRef] [PubMed]
    [Google Scholar]
  27. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39: 783– 791 [CrossRef] [PubMed]
    [Google Scholar]
  28. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16: 111– 120 [CrossRef] [PubMed]
    [Google Scholar]
  29. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  30. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970;12: 133– 142 [CrossRef] [PubMed]
    [Google Scholar]
  31. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983;4: 184– 192 [CrossRef] [PubMed]
    [Google Scholar]
  32. Wayne LG, Brennerdj CRR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987;37: 463– 464
    [Google Scholar]
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Microbacterium bovistercoris sp. nov., a novel actinomycete isolated from cow dung
Microbiology Society , ijsem003511; https://doi.org/10.1099/ijsem.0.003511
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