1887

Abstract

A Gram-stain-positive, aerobic actinomycete strain, designated NEAU-LLE, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, north-east China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLE belonged to the genus , with highest sequence similarity to DSM 22185 (98.2 %). The predominant menaquinones were MK-13 and MK-14. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid. The major fatty acids were identified as anteiso-C, iso-C and iso-C. However, DNA–DNA relatedness and physiological and biochemical data showed that strain NEAU-LLE could be distinguished from its closest relative. Therefore, strain NEAU-LLE represents a novel species of the genus , for which the name sp. nov is proposed, with NEAU-LLE (=CCTCC AA 2018025=JCM 32662) as the type strain.

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2019-09-01
2019-09-22
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