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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 9

sp. nov., a novel actinomycete isolated from cow dung Free

Abstract

A Gram-stain-positive, aerobic actinomycete strain, designated NEAU-LLE, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, north-east China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLE belonged to the genus , with highest sequence similarity to DSM 22185 (98.2 %). The predominant menaquinones were MK-13 and MK-14. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid. The major fatty acids were identified as anteiso-C, iso-C and iso-C. However, DNA–DNA relatedness and physiological and biochemical data showed that strain NEAU-LLE could be distinguished from its closest relative. Therefore, strain NEAU-LLE represents a novel species of the genus , for which the name sp. nov is proposed, with NEAU-LLE (=CCTCC AA 2018025=JCM 32662) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Microbacterium bovistercoris sp. nov. and polyphasic analysis
© 2019 IUMS
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2019-09-01
2024-04-19
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Microbacterium bovistercoris sp. nov., a novel actinomycete isolated from cow dung
Int J Syst Evol Microbiol 69, 2703 (2019); https://doi.org/10.1099/ijsem.0.003511
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