RT Journal Article SR Electronic(1) A1 Blackburn, Michael B. A1 Farrar Jr., Robert R. A1 Sparks, Michael E. A1 Kuhar, Daniel A1 Mowery, Joseph D. A1 Mitchell, Ashaki A1 Gundersen-Rindal, Dawn E.YR 2019 T1 Chromobacterium phragmitis sp. nov., isolated from estuarine marshes JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 9 SP 2681 OP 2686 DO https://doi.org/10.1099/ijsem.0.003508 PB Microbiology Society, SN 1466-5034, AB Thirteen isolates of Gram-stain-negative, motile, violet-pigmented bacteria were isolated from marshes along tidal portions of the Potomac and James rivers in Maryland and Virginia, USA, respectively. 16S rRNA gene sequences and fatty acid analysis revealed a high degree of relatedness among the isolates, and genomic sequencing of two isolates, IIBBL 112-1T and IIBBL 274-1 (from the Potomac and James rivers, respectively), revealed highly similar genomic sequences, with a blast-based average nucleotide identity (ANIb) of ca. 98.7 %. Phylogenetic analysis of 16S rRNA gene sequences suggested that the species most highly related to IIBBL 112-1T were Chromobacterium amazonense , Chromobacterium subtsugae and Chromobacterium sphagni . However, deletion of a 25-nucleotide sequence that may have been horizontally acquired by both IIBBL 112-1T and C. amazonense resulted in a substantially different analysis; in the latter case, the species nearest IIBBL 112-1T were Chromobacterium violaceum , Chromobacterium vaccinii and Chromobacterium piscinae . Whole-genome alignments between either IIBBL 112-1T or IIBBL 274-1 and the type strains of C. vaccinii or C. violaceum resulted in ANIb values in the range of ca. 87 %, while alignment with C. amazonense CBMAI 310T resulted in an ANIb of ca. 83 %. Collectively, these data demonstrate that IIBBL 112-1T and IIBBL 274-1 represent a new taxon within the genus Chromobacterium . We propose the name Chromobacterium phragmitis sp. nov. for this taxon; the type strain is IIBBL 112-1T (=NRRL B-67132T=JCM 31884T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003508