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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 8

sp. nov., an actinobacterium isolated from the wheat rhizosphere Free

Abstract

A novel actinobacterium, designated strain 1AS2c, was isolated from the wheat rhizosphere collected from the Brazilian biome. A polyphasic approach, including phenotypic characterization and phylogenetic multilocus sequence analysis (MLSA), was used to determine the taxonomic position of this strain. Analysis of the 16S rRNA gene sequence indicated that the novel strain is closely related to NBRC 13026, NBRC 13465 and ASBV-1, sharing a similarity value of 98.2, 98.1 and 97.9 %, respectively. Additionally, MLSA of five housekeeping genes (, , , and ) showed evolutionary distances beyond the 0.007 threshold, as well as low DNA–DNA relatedness between strain 1AS2c and its closest phylogenetic neighbours ( NBRC 13026, NBRC 13465 and ASBV-1: 56, 62.5 and 63.0 %, respectively), indicating a new phylogenetic lineage. The phylogenetic, chemotaxonomic and phenotypic characteristics support the assignment of strain 1AS2c to the genus , representing a novel species. It is concluded that strain 1AS2c (=CMAA 1679=NRRL B-65479=DSM 105299) can be classified as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): polyphasic approaches , Streptomyces rhizosphaericola sp. nov. and wheat rhizosphere
© 2019 IUMS
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2019-08-01
2024-04-18
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Streptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere
Int J Syst Evol Microbiol 69, 2431 (2019); https://doi.org/10.1099/ijsem.0.003498
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