@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003486, author = "Semenova, Ekaterina M. and Sokolova, Diyana S. and Grouzdev, Denis S. and Poltaraus, Andrey B. and Vinokurova, Natalia G. and Tourova, Tatiyana P. and Nazina, Tamara N", title = "Geobacillus proteiniphilus sp. nov., a thermophilic bacterium isolated from a high-temperature heavy oil reservoir in China", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "10", pages = "3001-3008", doi = "https://doi.org/10.1099/ijsem.0.003486", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003486", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "phylogenomic analysis", keywords = "oilfield", keywords = "polyphasic taxonomy", keywords = "Geobacillus proteiniphilus sp. nov.", abstract = "A rod-shaped, spore-forming, thermophilic, chemoorganotrophic, aerobic or facultatively anaerobic bacterial strain, 1017T, was isolated from production water sampled at the Dagang oilfield (PR China), and was characterized by using a polyphasic approach. The strain is capable of anaerobic glucose fermentation. Nitrate is reduced to nitrite. Optimal growth was observed at 60–65 °C, at pH between pH 7.0 and 7.5, and with 1–2 % (w/v) NaCl. The major cellular fatty acids were iso-C17 : 0, anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0. The predominant polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analysis based on the 16S rRNA, gyrB and parE gene sequences indicated that the isolate belonged to the genus Geobacillus and was most closely related to Geobacillus thermoleovorans KCTC 3570T (99.5, 96.1 and 97.9 % sequence similarity, respectively). Genome sequencing revealed a genome size of 3.57495 Mb and a DNA G+C content of 51.8 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between the genomes of strain 1017T and G. thermoleovorans KCTC 3570T were 95.9 and 64.9 %, respectively. Results of phylogenomic metrics analysis of the genome and 1172 core genes of strain 1017T and its physiological and biochemical characteristics confirmed that strain 1017T represented a novel species of the genus Geobacillus , for which the name Geobacillus proteiniphilus sp. nov. is proposed. The type strain is 1017T (=VKM B-3132T=KCTC 33986T).", }