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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 10

sp. nov., a thermophilic bacterium isolated from a high-temperature heavy oil reservoir in China Free

Abstract

A rod-shaped, spore-forming, thermophilic, chemoorganotrophic, aerobic or facultatively anaerobic bacterial strain, 1017, was isolated from production water sampled at the Dagang oilfield (PR China), and was characterized by using a polyphasic approach. The strain is capable of anaerobic glucose fermentation. Nitrate is reduced to nitrite. Optimal growth was observed at 60–65 °C, at pH between pH 7.0 and 7.5, and with 1–2 % (w/v) NaCl. The major cellular fatty acids were iso-C, anteiso-C, iso-C, iso-C and C. The predominant polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analysis based on the 16S rRNA, and gene sequences indicated that the isolate belonged to the genus and was most closely related to KCTC 3570 (99.5, 96.1 and 97.9 % sequence similarity, respectively). Genome sequencing revealed a genome size of 3.57495 Mb and a DNA G+C content of 51.8 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between the genomes of strain 1017 and KCTC 3570 were 95.9 and 64.9 %, respectively. Results of phylogenomic metrics analysis of the genome and 1172 core genes of strain 1017 and its physiological and biochemical characteristics confirmed that strain 1017 represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1017 (=VKM B-3132=KCTC 33986).

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  • Accepted:
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Keyword(s): Geobacillus proteiniphilus sp. nov. , oilfield , phylogenomic analysis and polyphasic taxonomy
© 2019 IUMS
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2019-10-01
2024-04-24
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Geobacillus proteiniphilus sp. nov., a thermophilic bacterium isolated from a high-temperature heavy oil reservoir in China
Int J Syst Evol Microbiol 69, 3001 (2019); https://doi.org/10.1099/ijsem.0.003486
/content/journal/ijsem/10.1099/ijsem.0.003486
/content/journal/ijsem/10.1099/ijsem.0.003486
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