1887

Abstract

Two conspecific yeast strains, which based on DNA sequence comparisons represented an undescribed species in the order Trichosporonales were isolated during two independent studies in Hungary and France. One of them (NCAIM Y.02224) was recovered from minced pork in Hungary while the other one (UBOCC-A-218003) was isolated from the air of a dairy plant in France. The two strains shared identical nucleotide sequences in the D1/D2 domain of the nuclear large subunit (LSU) rRNA gene and in the internal transcribed spacer (ITS) region. Analysis of the concatenated DNA sequences for the ITS region and D1/D2 domain of the LSU rRNA gene indicated that the novel species belongs to the recently erected genus . According to sequence comparisons and phylogenetic analysis, the novel species is most closely related to (formerly ), which is often associated with humans and warm-blooded animals. The physiological characteristics of this novel species are also very similar to that of . The only clear-cut difference is that, unlike , the novel species does not utilize imidazole as a nitrogen-source. The species name sp. nov. is proposed to accommodate the above-noted two strains.

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2019-08-01
2024-04-19
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References

  1. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 2015; 81:85–147 [View Article][PubMed]
    [Google Scholar]
  2. Liu XZ, Wang QM, Theelen B, Groenewald M, Bai FY et al. Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 2015; 81:1–26 [View Article][PubMed]
    [Google Scholar]
  3. Sugita T. Trichosporon Behrend (1890). Yeasts: A Taxonomic Study, 5th ed. vol. 1-3 2011 pp. 2015–2061
    [Google Scholar]
  4. Takashima M, Sriswasdi S, Manabe RI, Ohkuma M, Sugita T et al. A Trichosporonales genome tree based on 27 haploid and three evolutionarily conserved 'natural' hybrid genomes. Yeast 2018; 35:99–111 [View Article][PubMed]
    [Google Scholar]
  5. Sugita T, Takashima M, Sano A, Nishimura K, Kinebuchi T et al. Cryptococcus arboriformis sp. nov., a novel anamorphic basidiomycetous yeast species isolated from a patient's urine. Microbiol Immunol 2007; 51:543–545 [View Article][PubMed]
    [Google Scholar]
  6. Fonseca A, Boekhout T, Fell JW. Cryptococcus Vuillemin (1901). Yeasts: A Taxonomic Study, 5th ed. vol. 1-3 2011 pp. 1661–1737
    [Google Scholar]
  7. Motaung TE, Albertyn J, Kock JL, Pohl CH. Cryptococcus cyanovorans sp. nov., a basidiomycetous yeast isolated from cyanide-contaminated soil. Int J Syst Evol Microbiol 2012; 62:1208–1214 [View Article][PubMed]
    [Google Scholar]
  8. Garnier L, Valence F, Pawtowski A, Auhustsinava-Galerne L, Frotté N et al. Diversity of spoilage fungi associated with various French dairy products. Int J Food Microbiol 2017; 241:191–197 [View Article][PubMed]
    [Google Scholar]
  9. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. Yeasts: A Taxonomic Study, 5th ed. vol. 1-3 2011 pp. 87–110
    [Google Scholar]
  10. Marquina D, Peres C, Caldas FV, Marques JF, Peinado JM et al. Characterization of the yeast population in olive brines. Lett Appl Microbiol 1992; 14:279–283 [View Article]
    [Google Scholar]
  11. Nagy E, Dlauchy D, Medeiros AO, Péter G, Rosa CA. Yarrowia porcina sp. nov. and Yarrowia bubula f.a. sp. nov., two yeast species from meat and river sediment. Antonie van Leeuwenhoek 2014; 105:697–707 [View Article]
    [Google Scholar]
  12. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
    [Google Scholar]
  13. Péter G, Tornai-Lehoczki J, Dlauchy D. Candida ogatae sp. nov., an anamorphic member of the Kuraishia clade. FEMS Yeast Res 2009; 9:328–333 [View Article][PubMed]
    [Google Scholar]
  14. Zhang Z, Schwartz S, Wagner L, Miller W. A greedy algorithm for aligning DNA sequences. J Comput Biol 2000; 7:203–214 [View Article][PubMed]
    [Google Scholar]
  15. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10:512–526 [View Article][PubMed]
    [Google Scholar]
  16. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article][PubMed]
    [Google Scholar]
  17. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  18. Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 2000; 50:1351–1371 [View Article][PubMed]
    [Google Scholar]
  19. Vu D, Groenewald M, Szöke S, Cardinali G, Eberhardt U et al. DNA barcoding analysis of more than 9000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 2016; 85:91–105 [View Article][PubMed]
    [Google Scholar]
  20. Daniel HM, Vrancken G, Takrama JF, Camu N, De Vos P et al. Yeast diversity of Ghanaian cocoa bean heap fermentations. FEMS Yeast Res 2009; 9:774–783 [View Article][PubMed]
    [Google Scholar]
  21. Nash AK, Auchtung TA, Wong MC, Smith DP, Gesell JR et al. The gut mycobiome of the human microbiome project healthy cohort. Microbiome 2017; 5:153 [View Article][PubMed]
    [Google Scholar]
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