@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003476, author = "Wang, Chong and Zhang, Rui and Liu, Bang-Tao and Liu, Chun-Li and Du, Zong-Jun", title = "Paracnuella aquatica gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a hot spring", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "8", pages = "2360-2366", doi = "https://doi.org/10.1099/ijsem.0.003476", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003476", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "phylogenetic analysis", keywords = "16s rRNA gene", keywords = "Paracnuella aquatica", abstract = "A Gram-stain-negative, rod-shaped bacterium (designated strain N24T) with gliding motility was isolated from thermal spring water sampled at Xi’an, PR China. Cells were 0.4–0.8 µm wide and 1.8–7.8 µm long. Optimal growth occurred at 33 °C and pH 7.0 on Reasoner's 2A (R2A) agar. Strain N24T could produce exopolysaccharide on R2A agar at 33 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain N24T formed a distinct phyletic lineage within the family Chitinophagaceae and was most closely related to members of the genera Flavisolibacter , Cnuella , Niveitalea , Flavitalea , Flaviaesturariibacter and Niastella with 91.7–93.9 % 16S rRNA gene sequence similarities. The major fatty acids of strain N24T were iso-C15 : 0 (31.8 %), iso-C17 : 0 3-OH (16.1 %) and iso-C15 : 1 G (12.9 %). The polar lipid profile consisted of phosphatidylethanolamine, two aminolipids and six unknown lipids; the quinone system consisted of menaquinone-7 (MK-7). The genomic G+C content was 49.3 mol%. On the basis of the evidence presented in this study, strain N24T represents a novel species of a new genus in the family Chitinophagaceae , for which the name Paracnuella aquatica gen. nov., sp. nov. is proposed. The type species is N24T (=KCTC 62083T=MCCC 1H00301T).", }