1887

Abstract

A Gram-stain-negative, rod-shaped bacterium (designated strain N24) with gliding motility was isolated from thermal spring water sampled at Xi’an, PR China. Cells were 0.4–0.8 µm wide and 1.8–7.8 µm long. Optimal growth occurred at 33 °C and pH 7.0 on Reasoner's 2A (R2A) agar. Strain N24 could produce exopolysaccharide on R2A agar at 33 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain N24 formed a distinct phyletic lineage within the family and was most closely related to members of the genera , , , , and with 91.7–93.9 % 16S rRNA gene sequence similarities. The major fatty acids of strain N24 were iso-C (31.8 %), iso-C 3-OH (16.1 %) and iso-C G (12.9 %). The polar lipid profile consisted of phosphatidylethanolamine, two aminolipids and six unknown lipids; the quinone system consisted of menaquinone-7 (MK-7). The genomic GC content was 49.3 mol%. On the basis of the evidence presented in this study, strain N24 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type species is N24 (=KCTC 62083=MCCC 1H00301).

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2019-08-01
2019-10-14
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