1887

Abstract

A novel Gram-stain-positive, strictly aerobic, motile, spore-forming and rod-shaped bacterial strain, designated R-3, was isolated from a soil sample obtained from the shore of Lake Panyang, Sichuan Province, PR China. Strain R-3 hydrolysed starch and casein. It could not assimilate -glucose as a carbon source, or produce acid from -glucose and -arabinose. Phylogenetic, phenotypic, chemotaxonomic and molecular studies were performed on the new isolate. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain R-3 was a member of the genus , exhibiting the highest sequence similarity to TEGR-3 (98.4 %). The organism grew at 4–38 °C (optimum, 28–30 °C), at pH 6.0–10.0 (pH 7.0–7.5) and with 0–2.5 % (w/v) NaCl (1 %). The predominant menaquinone was MK-7. Anteiso-C (60.7 %) and C (15.5 %) were the major fatty acids. The cellular polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified phospholipid. The DNA G+C content of strain R-3 was determined to be 47.0 mol%. The DNA–DNA relatedness between strain R-3 and TEGR-3 was 21.2 %. Based on the results obtained in this study, strain R-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R-3 (=CGMCC 1.16135=KCTC 33912).

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2019-08-01
2019-08-25
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