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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 8

sp. nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano Free

Abstract

strain HST28 isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study. Strain HST28 showed morphological and chemotaxonomic features in line with its classification in the genus Optimal growth of strain HST28 was obtained at 28 °C, pH 8–9 and up to 10 % (w/v) NaCl. Single (16S rRNA) and multi-locus gene sequence analyses showed that strain HST28 had a distinct phylogenetic position from its closest relatives, the type strains of and . Digital DNA–DNA hybridization (23.3 and 31.0 %) and average nucleotide identity (79.3 and 85.6 %) values between strain HST28 and its corresponding relatives mentioned above were below the threshold of 70 and 96 %, respectively, defined for assigning a prokaryotic strains to the same species. Strain HST28 was characterised by the presence of -diaminopimelic acid in its peptidoglycan layer; galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars; phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid as polar lipids; and the predominating menaquinones MK-9(H), MK-9(H) and MK-9(H4) (>20 %) as well as anteiso-C and anteiso-C as major fatty acids (>15 %). Based on the phenotypic and genetic results, strain HST28 (DSM 107268=CECT 9648) merits recognition as a new species named sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): phenotyping , phylogeny , polyphasic taxonomy and systematic
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2019-08-01
2023-09-28
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Streptomyces huasconensis sp. nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano
Int J Syst Evol Microbiol 69, 2315 (2019); https://doi.org/10.1099/ijsem.0.003468
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