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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 8

Reclassification of as a later heterotypic synonym of based on whole-genome sequence analysis Free

Abstract

The genus belongs to the family and its members are known to be adapted to a wide range of ecological niches. ATCC 49188 and LUP21 are strains isolated from human clinical and plant root nodule samples, respectively, which share high similarity for phylogenetic markers (i.e 100 % for 16S rRNA, 99.9 % for and 99.35 % for ). In this work, multiple genome average nucleotide identity (ANI) approaches, digital DNA–DNA hybridization (dDDH) and phylogenetic analysis were performed in order to investigate the taxonomic relationship between ATCC 49188, LUP21, and other five type strains from the genus . Whole-genome comparisons demonstrated that LUP21 and the genus type species ATCC 49188, share 97.55 % of ANIb, 98.25 % of ANIm, 97.99 % of gANI, 97.94 % of OrthoANI and 83.9 % of dDDH, which exceed the species delineation thresholds. These strains are also closely related in phylogenies reconstructed from a concatenation of 1193 sequences from single-copy ortholog genes. A review of their profiles revealed that ATCC 49188 and LUP21 do not present pronounced differences at phenotypic and chemotaxonomic levels. Considering phylogenetic, genomic, phenotypic and chemotaxonomic data, should be considered a later heterotypic synonym of .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ANI , dDDH , genome-based taxonomy , Phylogenomics and rhizobia
© 2019 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.003465
2019-08-01
2024-04-23
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