1887

Abstract

A single bacterial isolate, EPLC-04, was isolated from human feces and identified as representing a member of the genus on the basis of phenotypic characteristics, matrix assisted laser desorption ionization time-of-flight mass spectrometry and partial 16S rRNA gene sequencing. The isolate’s phenotypic profile differed from that described for the most closely related species, , by exhibiting lipase production and lacking pyrazinamidase activity. Multiple genetic targets, including the complete (1465 bp) 16S rRNA gene sequence and partial sequences of (539 bp), (935 bp), (525 bp) and (535 bp) indicated that the isolate exhibited 98.91, 92.16, 90.81, 92.78 and 89.01 % identity with , 98.98, 91.99, 90.17, 89.77 and 89.55 % identity with , and 99.66, 98.11, 98.50, 98.49 and 98.51 % identity with , respectively. Phylogenetic reconstructions based on the combination of the four housekeeping genes indicated that the isolate formed a unique branch, supported by a bootstrap value of 100 %. Digital DNA–DNA homology and 16S rRNA gene sequencing identified EPLC-04 as representing . However, the unique phenotypic traits and results of phylogenetic analysis indicate that it represents a novel subspecies of . The name subsp. subsp. nov. is proposed for this novel taxon (type strain EPLC-04=ATCC BAA-2637, DSMZ 28595).

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2019-08-01
2019-10-14
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