RT Journal Article SR Electronic(1) A1 Heo, Jun A1 Cho, Hayoung A1 Tamura, Tomohiko A1 Saitou, Satomi A1 Park, Kwanho A1 Kim, Jeong-Seon A1 Hong, Seung-Beom A1 Kwon, Soon-Wo A1 Kim, Soo-JinYR 2019 T1 Lactococcus allomyrinae sp. nov., isolated from gut of larvae of Allomyrina dichotoma JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 12 SP 3682 OP 3688 DO https://doi.org/10.1099/ijsem.0.003461 PB Microbiology Society, SN 1466-5034, AB Strain 1JSPR-7T, a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7T fell within the genus Lactococcus , forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis . The 16S rRNA gene sequence of strain 1JSPR-7T revealed the highest homology with L. lactis subsp. lactis JCM 5805T (97.3 %) and L. lactis subsp. hordniae NBRC 100931T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactis subsp. cremoris DSM 20069T (91.4 %) and L. lactis subsp. tructae L105T (91.4 %). The average nucleotide identity and digital DNA–DNA hybridization values indicated that strain 1JSPR-7T was a novel species of the genus Lacococcus. The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C19 : 1ω6c and/or C19 : 0cyclo ω10c), C16 : 0 and C14 : 0, and the predominant menaquinone was MK-8 with MK-7 as a minor one. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one. The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp. The DNA G+C content based on the whole genome sequences was 37.4 mol%. Based on the data obtained, strain 1JSPR-7T represents a novel species of the genus Lactococcus , for which the name Lactococcus allomyrinae sp. nov. is proposed. The type strain is 1JSPR-7T (=KACC 19319T=NBRC 113068T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003461