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Abstract

Strain 1JSPR-7, a facultatively anaerobic bacterium isolated from the gut of larvae of raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene and gene sequences showed that strain 1JSPR-7 fell within the genus , forming a compact cluster with the type strain of four subspecies of and . The 16S rRNA gene sequence of strain 1JSPR-7 revealed the highest homology with subsp. JCM 5805 (97.3 %) and subsp. NBRC 100931 (97.1 %), and the gene sequence showed the highest similarity to subsp. DSM 20069 (91.4 %) and subsp. L105 (91.4 %). The average nucleotide identity and digital DNA–DNA hybridization values indicated that strain 1JSPR-7 was a novel species of the genus . The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, Cω6 and/or Ccyclo ω10), C and C, and the predominant menaquinone was MK-8 with MK-7 as a minor one. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one. The cell-wall peptidoglycan was of the A4 type with an interpeptide bridge comprising -Lys--Asp. The DNA G+C content based on the whole genome sequences was 37.4 mol%. Based on the data obtained, strain 1JSPR-7 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1JSPR-7 (=KACC 19319=NBRC 113068).

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2019-12-01
2021-07-28
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