RT Journal Article SR Electronic(1) A1 Benga, Laurentiu A1 Spröer, Cathrin A1 Schumann, Peter A1 Verbarg, Susanne A1 Bunk, Boyke A1 Engelhardt, Eva A1 Benten, W. Peter M. A1 Sager, MartinYR 2019 T1 Leucobacter muris sp. nov., isolated from the nose of a laboratory mouse JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 7 SP 2095 OP 2100 DO https://doi.org/10.1099/ijsem.0.003446 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, rod-shaped, aerobic, non-motile, white, opaque bacterial isolate, designated 924/12T, was isolated from the nose of a laboratory mouse in Düsseldorf, Germany. The 16S rRNA gene sequence analyses indicated the phylogenetic position of the strain within the genus Leucobacter . Similarity levels over 97 % were recorded between the 16S rRNA gene sequence of strain 924/12T and the type strains of the species Leucobacter chironomi DSM 19883T (99.5 %), followed by Leucobacter celer subsp. astrifaciens CBX151T (97.6 %), Leucobacter celer subsp. celer NAL101T (97.5 %), ‘ Leucobacter kyeonggiensis ’ F3-P9 (97.5 %), Leucobacter zeae CC-MF41T (97.3 %), Leucobacter chromiiresistens JG31T (97.1 %), Leucobacter triazinivorans JW-1T (97.1 %), Leucobacter corticis 2 C-7T (97.0 %) and Leucobacter aridicolis CIP108388T (97.0 %). DNA–DNA hybridization and whole genomic comparison, mandatory to taxonomically separate strain 924/12T from the type strain of L. chironomi , revealed similarity values of 40.4 and 30.8 %, respectively, thus below the threshold of 70 % recommended differentiating between species. The cell-wall amino acids of the novel isolate were diaminobutyric acid, alanine, glycine, threonine and glutamic acid. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unknown lipid, whereas the predominant menaquinones were MK-11 and MK-10. The genomic DNA G+C content of strain 924/12T was 70.6 mol%. Phylogenetic analyses based on the 16S rRNA gene sequences and the phenotypical differences between strain 924/12T and the other closely related type strains of the genus Leucobacter indicated that strain 924/12T represents a novel species within the genus Leucobacter , family Microbacteriaceae , for which the name Leucobacter muris sp. nov. is proposed. The type strain is 924/12T (=DSM 101948T=CCM 8761T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003446