1887

Abstract

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain X7X, was isolated from a rhizosphere soil sample of Nicotiana tabacum L. collected from a tobacco factory located in Kunming, south-western China. The cells showed oxidase-positive and catalase-positive reactions. Growth occurred at 20–40 °C and pH 6.0–8.0, with optimal growth at 30 °C and pH 7.0. The predominant respiratory quinone was MK-7. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 3OH and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). The cellular polar lipids contained phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified glycolipids, four unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 49.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain X7X should be affiliated to the genus Flavisolibacter . Results from further analysis showed that strain X7X had highest 16S rRNA gene sequence similarity to Flavisolibacter metallilatus TX0661 (96.4 %) and ‘ Flavisolibacter swuensis ’ SR2-4-2 (96.4 %), followed by other species of the genus Flavisolibacter . The polyphasic taxonomic characteristics indicated that strain X7X represents a novel species of the genus Flavisolibacter , for which the name Flavisolibacter nicotianae sp. nov. (type strain X7X=KCTC 62326=CGMCC 16451) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003440
2019-05-17
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/7/2082.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003440&mimeType=html&fmt=ahah

References

  1. Yoon MH, Im WT, Wt I. Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. Int J Syst Evol Microbiol 2007; 57:1834–1839 [View Article][PubMed]
    [Google Scholar]
  2. Baik KS, Kim MS, Lee JH, Lee SS, Im WT et al. Flavisolibacter rigui sp. nov., isolated from freshwater of an artificial reservoir and emended description of the genus Flavisolibacter . Int J Syst Evol Microbiol 2014; 64:4038–4042 [View Article][PubMed]
    [Google Scholar]
  3. Lee JJ, Kang MS, Kim GS, Lee CS, Lim S et al. Flavisolibacter tropicus sp. nov., isolated from tropical soil. Int J Syst Evol Microbiol 2016; 66:3413–3419 [View Article][PubMed]
    [Google Scholar]
  4. Kim DU, Lee H, Lee S, Kim SG, Park AY et al. Flavisolibacter metallilatus sp. nov., isolated from an automotive air conditioning system and emended description of the genus Flavisolibacter . Int J Syst Evol Microbiol 2018; 68:917–923 [View Article][PubMed]
    [Google Scholar]
  5. Zhao Y, Liu Q, Kang MS, Jin F, Yu H et al. Flavisolibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity isolated from soil used for cultivating ginseng. Int J Syst Evol Microbiol 2015; 65:4868–4872 [View Article][PubMed]
    [Google Scholar]
  6. Reasoner DJ, Geldreich EE. A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 1985; 49:1–7[PubMed]
    [Google Scholar]
  7. Skerman VBD. A Guide to the Identification of the Genera of Bacteria, 2nded. Baltimore: Williams & Wilkins; 1967
    [Google Scholar]
  8. Gregersen T. Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 1978; 5:123–127 [View Article]
    [Google Scholar]
  9. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  10. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington: American Society for Microbiology; 1994 pp. 607–655
    [Google Scholar]
  11. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
    [Google Scholar]
  12. Groth I, Schumann P, Weiss N, Martin K, Rainey FA. Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 1996; 46:234–239 [View Article][PubMed]
    [Google Scholar]
  13. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
    [Google Scholar]
  14. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  15. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI technical note 101. Newwark, DE: MIDI Inc; 1990
    [Google Scholar]
  16. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  17. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1618 [View Article][PubMed]
    [Google Scholar]
  18. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  19. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  20. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  21. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  22. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  23. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  24. Luo R, Liu B, Xie Y, Li Z, Huang W et al. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 2012; 1:18 [View Article][PubMed]
    [Google Scholar]
  25. Delcher AL, Harmon D, Kasif S, White O, Salzberg SL. Improved microbial gene identification with GLIMMER. Nucleic Acids Res 1999; 27:4636–4641 [View Article][PubMed]
    [Google Scholar]
  26. Joo ES, Cha S, Kim MK, Jheong W, Seo T et al. Flavisolibacter swuensis sp. nov. isolated from soil. J Microbiol 2015; 53:442–447 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003440
Loading
/content/journal/ijsem/10.1099/ijsem.0.003440
Loading

Data & Media loading...

Supplements

Supplementary data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error