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Abstract

A Gram-stain-positive, aerobic, coccoid-shaped, non-spore-forming actinobacterial strain, designated M5W7-7, was isolated from a hot spring soil sample collected from Anshan, Liaoning province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M5W7-7 clustered closely with species of the genus Kocuria , and showed the highest sequence similarity of 97.1 % to Kocuria subflava YIM 13062. Strain M5W7-7 grew at 10–37 °C (optimum, 37 °C), pH6.0–11.0 (pH 6.0–7.0) and in the presence of 0–7 % (w/v) NaCl (0 %). Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested. Strain M5W7-7 contained MK-6(H2) and MK-7(H2) as the dominant menaquinones. The polar lipid profile of strain M5W7-7 contained diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. The predominant whole-cell sugars were galactose and glucose. The predominant fatty acid was anteiso-C15 : 0. The DNA G+C content of strain M5W7-7 was 67.0 mol%. On the basis of phylogenetic relationships, phenotypic characterization and chemotaxonomic analyses, strain M5W7-7 represents a novel species of the genus Kocuria , for which the name Kocuria soli sp. nov. is proposed. The type strain is M5W7-7 (=KCTC 49195 =CGMCC 1.13744).

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2019-05-15
2019-08-19
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