%0 Journal Article %A Huo, Yunyun %A Tong, Wenjun %A Wang, Juanjuan %A Wang, Fang %A Bai, Wenqing %A Wang, Entao %A Shi, Peng %A Chen, Weimin %A Wei, Gehong %T Rhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China %D 2019 %J International Journal of Systematic and Evolutionary Microbiology, %V 69 %N 7 %P 2049-2056 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003430 %K ANI %K dDDH %K rhizobium %K Phaseolus vulgaris L. %K 16S rRNA %I Microbiology Society, %X Two Gram-stain-negative, rod-shaped bacterial strains (C5T and C16), isolated from root nodules of Phaseolus vulgaris L. in Jiangxi Province, PR China, were characterized by using a polyphasic taxonomical approach. The phylogenetic analysis of the 16S rRNA gene and three concatenated housekeeping genes (recA–glnII–atpD) revealed that C5T and C16 were members of the genus Rhizobium , yet were distinct from known species. The case for strain C5T representing a novel species was supported by genomic results. Pairwise digital DNA–DNA hybridization and average nucleotide identity values were much lower than the proposed and generally accepted species boundaries. The genome-based phylogenetic tree reconstructed by using the up-to-date bacterial core gene set consisting of 92 genes showed that the strains formed a monophyletic branch, further supporting this result. The symbiotic genes of nodC and nifH were identified in both strains; each could nodulate Phaseolus vulgaris and Glycine max but not Leucaena leucocephala, Pisum sativum or Medicago sativa plants. Major cellular fatty acids of C5T were summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c; 58.8 %), C18 : 1 ω7c 11-methyl (14.2 %) and C18 : 0 (8.1 %). The DNA G+C content of C5T was 61.4 mol%. Based on these genomic, chemotaxonomic and phenotypic characteristics, we propose a novel species: Rhizobium chutanense sp. nov. The type strain is C5T (=CCTCC AB 2018143T=LMG 30777T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003430