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Abstract

Strain KYPY4, isolated from a water sample taken from the Funglin stream in Taiwan, was characterized using a polyphasic taxonomic approach. The 16S rRNA gene sequence of strain KYPY4 was closely related to CCUG 30898 (97.1 %) followed by L10 (96.9 %) and KIN192 (96.8 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KYPY4 belonged to the genus and formed a distinct phyletic line with L10 and TTM-94. The draft genome of strain KYPY4 was approximately 4.93 Mb in size with a G+C content of 70.1 mol%. Strain KYPY4 showed 73.4–76.9 % average nucleotide identity and 21.3–22.5 % digital DNA–DNA hybridization identity with the strains of other related species in the group. Cells of strain KYPY4 were Gram-stain-negative, aerobic, motile, surrounded by a thick capsule and formed light orange colonies. Optimal growth occurred at 30 °C, pH 7 and 0 % NaCl. Strain KYPY4 contained summed feature 3 (comprising Cω7 and/or Cω6), C and Cω7 as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized polar lipids. The major respiratory quinone was Q-8. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain KYPY4 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KYPY4 (=BCRC 81010=KCTC 52441).

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2019-08-01
2019-08-19
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