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Abstract

A taxonomic study was carried out on strain HN-Y73, which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Strain HN-Y73 was Gram-stain-negative, non-motile, ovoid- to rod-shaped, yellow-pigmented and could grow at 10–42 °C (optimum, 28 °C), at pH 5.0–9.0 (pH 8.0) and in 0.5–16 % (w/v) NaCl (3–4 %). This isolate was positive for oxidase, catalase and the hydrolysis of aesculin and xylan, but negative for the hydrolysis of casein and gelatin. Strain HN-Y73 shared the highest 16S rRNA gene sequence similarity with Altererythrobacter endophyticus BR75 (96.9 %), followed by Altererythrobacter estronivorus MH-B5 (96.0 %), Altererythrobacter indicus MSSRF26 (95.9 %) and Altererythrobacter mangrovi C9-11 (95.7 %). The phylogenetic tree showed that strain HN-Y73 forms a clade with A. endophyticus BR75, A. indicus MSSRF26 and A. estronivorus MH-B5 within the genus Altererythrobacter . The dominant fatty acids were summed feature 8 (C18 : 1ω7c/ω6c) and C19 : 0cyclo ω8c. The major polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, sphingoglycolipid phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, one unidentified glycolipid, two unidentified phospholipids and four unidentified lipids. The respiratory lipoquinone was identified as Q-10. The G+C content of the genomic DNA was 57.6 mol%. Based on the phenotypic and phylogenetic data, strain HN-Y73 represents a novel species of the genus Altererythrobacter , for which the name Altererythrobacter spongiae sp. nov. is proposed. The type strain is HN-Y73 (=MCCC 1K03324=LMG 30461).

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2019-07-01
2019-10-19
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References

  1. Kwon KK, Woo JH, Yang SH, Kang JH, Kang SG et al. Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov. Int J Syst Evol Microbiol 2007;57:2207–2211 [CrossRef][PubMed]
    [Google Scholar]
  2. Xue X, Zhang K, Cai F, Dai J, Wang Y et al. Altererythrobacter xinjiangensis sp. nov., isolated from desert sand, and emended description of the genus Altererythrobacter. Int J Syst Evol Microbiol 2012;62:28–32 [CrossRef][PubMed]
    [Google Scholar]
  3. Matsumoto M, Iwama D, Arakaki A, Tanaka A, Tanaka T et al. Altererythrobacter ishigakiensis sp. nov., an astaxanthin-producing bacterium isolated from a marine sediment. Int J Syst Evol Microbiol 2011;61:2956–2961 [CrossRef][PubMed]
    [Google Scholar]
  4. Jung YT, Park S, Lee JS, Yoon JH. Altererythrobacter aestiaquae sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2014;64:3943–3949 [CrossRef][PubMed]
    [Google Scholar]
  5. Jeong SH, Jin HM, Lee HJ, Jeon CO. Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 2013;63:971–976 [CrossRef][PubMed]
    [Google Scholar]
  6. Xue H, Piao CG, Guo MW, Wang LF, Fang W et al. Description of Altererythrobacter aerius sp. nov., isolated from air, and emended description of the genus Altererythrobacter. Int J Syst Evol Microbiol 2016;66:4543–4548 [CrossRef][PubMed]
    [Google Scholar]
  7. Fidalgo C, Rocha J, Martins R, Proença DN, Morais PV et al. Altererythrobacter halimionae sp. nov. and Altererythrobacter endophyticus sp. nov., two endophytes from the salt marsh plant Halimione portulacoides. Int J Syst Evol Microbiol 2017;67:3057–3062 [CrossRef][PubMed]
    [Google Scholar]
  8. Zhao Q, Li HR, Han QQ, He AL, Nie CY et al. Altererythrobacter soli sp. nov., isolated from desert sand. Int J Syst Evol Microbiol 2017;67:454–459 [CrossRef][PubMed]
    [Google Scholar]
  9. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–IN1 [CrossRef]
    [Google Scholar]
  10. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617
    [Google Scholar]
  11. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  14. Høvik Hansen G, Sørheim R. Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 1991;13:231–241 [CrossRef]
    [Google Scholar]
  15. Smibert RM. Phenotypic characterization. Methods for General and Molecular Bacteriology 1994
    [Google Scholar]
  16. Lemos ML, Toranzo AE, Barja JL. Modified medium for the oxidation-fermentation test in the identification of marine bacteria. Appl Environ Microbiol 1985;49:1541–1543[PubMed]
    [Google Scholar]
  17. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. 1990
  18. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  19. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977;100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  20. Groth I, Schumann P, Weiss N, Martin K, Rainey FA. Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 1996;46:234–239 [CrossRef][PubMed]
    [Google Scholar]
  21. Kumar NR, Nair S, Langer S, Busse HJ, Kämpfer P et al. Altererythrobacter indicus sp. nov., isolated from wild rice (Porteresia coarctata Tateoka). Int J Syst Evol Microbiol 2008;58:839–844 [CrossRef][PubMed]
    [Google Scholar]
  22. Qin D, Ma C, Hu A, Zhang F, Hu H et al. Altererythrobacter estronivorus sp. nov., an estrogen-degrading strain isolated from Yundang Lagoon of Xiamen City in China. Curr Microbiol 2016;72:634–640 [CrossRef][PubMed]
    [Google Scholar]
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