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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 7

Cohnella endophytica sp. nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala Free

Abstract

A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China. The bacterium was characterized by a polyphasic approach to determine its taxonomic position. 16S rRNA gene sequence comparisons revealed that strain M2MS4P-1 belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22R (98.4 % similarity). The average nucleotide identity value and estimated DDH value between strain M2MS4P-1 and the type strain of C. luojiensis HY-22R were 79.2 and 20.1 %, respectively. Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested. Strain M2MS4P-1 grew in the pH range 6.0–9.0 (optimum, pH 7.0–8.0), at temperatures between 10–37 °C (30 °C) and in 0–1 % (w/v) NaCl (0 %). The predominant isoprenoid quinone in strain M2MS4P-1 was menaquinone-7. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, four unidentified aminophospholipids and two unidentified phospholipids. The DNA G+C content was 51.5 mol%. According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1 was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnella endophytica sp. nov. The type strain is M2MS4P-1 (=KCTC 43011=CGMCC 1.13745).

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Keyword(s): Cohnella , endophytic bacterium , novel species and polyphasic approach
© 2019 IUMS
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2019-05-07
2024-04-24
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Cohnella endophytica sp. nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala
Int J Syst Evol Microbiol 69, 2004 (2019); https://doi.org/10.1099/ijsem.0.003417
/content/journal/ijsem/10.1099/ijsem.0.003417
/content/journal/ijsem/10.1099/ijsem.0.003417
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