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Abstract

A Gram-stain-positive, alkaliphilic, moderately halophilic, cocci-shaped actinobacterium (strain M8) was isolated from a sample of soda lake sediment (Lake Magadi, Tanzania). The isolate was heterotrophic, strictly aerobic, catalase-positive, oxidase-negative and formed orange-pigmented colonies in solid media. It utilized various sugars and organic acids as sole carbon sources. The organism grew at 10–38 °C, at pH 7.5–12.0 and in the presence of 1–12 % (w/v) NaCl, with optimal growth occurring at 30 °C, at pH 10 and in the presence of 5 % (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain M8 belonged to the genus Nesterenkonia , sharing the closest similarities to Nesterenkoniahalobia DSM 20541, Nesterenkoniahalophila YIM 70179 and Nesterenkoniaaethiopica DSM 17733 (97.5, 97.5 and 97.1 %, respectively). The characteristic diamino acid of strain M8 was found to be lysine and the polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. The DNA G+C content was 61.8 mol% (genome). The strain contained MK-7, MK-9 and MK-10 as the respiratory quinones, and the major fatty acids (>10 %) comprised anteiso-C17 : 0 and anteiso-C15 : 0. On the basis of phylogenetic analyses and phenotypic data, strain M8 is considered to represent a novel species, for which the name Nesterenkonia natronophila sp. nov. is proposed. The type strain is M8 (=JCM 32100=CGMCC 1.16706=MCC 3367).

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2019-05-02
2022-01-24
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