1887

Abstract

Two strictly anaerobic strains (MB11 and MB56) were isolated from common marmoset (Callithrixjacchus) faeces. Cells of the two strains were Gram-stain-negative, pleomorphic short (strain MB11) or long (strain MB56) rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates were related to the genus Phascolarctobacterium . They had 16S rRNA gene sequences similarities lower than 93 % to previously described species, Phascolarctobacterium faecium ACM 3679 and Phascolarctobacterium succinatutens YIT 12067, and 98.7 % between themselves. DNA–DNA hybridization values showed that strains MB11 and MB56 were the same species. The genomic DNA G+C content of strains MB11 and MB56 were 47.3–47.4 mol% and 47.7–48.0 mol%. The isolates had different enzymatic activities compared with P. succinatutens JCM 16074 and different major cellular fatty acids compared with P. faecium ACM 3679. Substrate availability revealed that they utilized not only succinate, but also pyruvate. With pyruvate supplementation, they produced both propionate and acetate, while only propionate production occured with succinate. As suggested by the phylogenic and physiological properties of strains MB11 and MB56, we propose the name Phascolarctobacterium wakonense sp. nov. with the type strain MB11 (=JCM 32899=DSM 107697).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003407
2019-04-30
2020-11-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/7/1941.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003407&mimeType=html&fmt=ahah

References

  1. Del Dot T, Osawa R, Stackebrandt E. Phascolarctobacterium faecium gen. nov, spec. nov., a novel taxon of the Sporomusa group of Bacteria. Syst Appl Microbiol 1993;16:380–384 [CrossRef]
    [Google Scholar]
  2. Marchandin H, Teyssier C, Campos J, Jean-Pierre H, Roger F et al. Negativicoccus succinicivorans gen. nov., sp. nov., isolated from human clinical samples, emended description of the family Veillonellaceae and description of Negativicutes classis nov., Selenomonadales ord. nov. and Acidaminococcaceae fam. nov. in the bacterial phylum Firmicutes. Int J Syst Evol Microbiol 2010;60:1271–1279 [CrossRef][PubMed]
    [Google Scholar]
  3. Osawa R, Fujisawa T, Mitsuoka T. Characterization of Gram-negative anaerobic strains, isolated from koala feces, which exhibit satellite growth and pleomorphism. Syst Appl Microbiol 1992;15:628–635 [CrossRef]
    [Google Scholar]
  4. Watanabe Y, Nagai F, Morotomi M. Characterization of Phascolarctobacterium succinatutens sp. nov., an asaccharolytic, succinate-utilizing bacterium isolated from human feces. Appl Environ Microbiol 2012;78:511–518 [CrossRef][PubMed]
    [Google Scholar]
  5. Paliy O, Kenche H, Abernathy F, Michail S. High-throughput quantitative analysis of the human intestinal microbiota with a phylogenetic microarray. Appl Environ Microbiol 2009;75:3572–3579 [CrossRef][PubMed]
    [Google Scholar]
  6. Rajilić-Stojanović M, De Vos WM. The first 1000 cultured species of the human gastrointestinal microbiota. FEMS Microbiol Rev 2014;38:996–1047 [CrossRef][PubMed]
    [Google Scholar]
  7. Wong JM, De Souza R, Kendall CW, Emam A, Jenkins DJ. Colonic health: fermentation and short chain fatty acids. J Clin Gastroenterol 2006;40:235–243 [CrossRef][PubMed]
    [Google Scholar]
  8. Reichardt N, Duncan SH, Young P, Belenguer A, McWilliam Leitch C et al. Phylogenetic distribution of three pathways for propionate production within the human gut microbiota. ISME J 2014;8:1323–1335 [CrossRef][PubMed]
    [Google Scholar]
  9. Mitsuoka T. Intestinal bacteriology, Asakura shoten, Tokyo, Japan, 1990 in Japanese.
  10. Holdeman L V, Cato EP, Moore WEC. Anaerobe Laboratory Manual, 4th ed. Blacksburg, VA: Virginia Polytechnic Institute and State University; 1977
    [Google Scholar]
  11. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  12. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  13. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547–1549 [CrossRef][PubMed]
    [Google Scholar]
  14. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  15. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  16. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68:461–466 [CrossRef][PubMed]
    [Google Scholar]
  17. Katayama-Fujimura Y, Komatsu Y, Kuraishi H, Kaneko T. Estimation of DNA base composition by high performance liquid chromatography if its nuclease Pl hydrolysate. Agric Biol Chem 1984;48:3169–3172
    [Google Scholar]
  18. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–IN1 [CrossRef]
    [Google Scholar]
  19. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used To determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003407
Loading
/content/journal/ijsem/10.1099/ijsem.0.003407
Loading

Data & Media loading...

Supplements

Supplementary data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error