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Abstract

A Gram-stain-negative, facultative anaerobic, red-coloured, rod-shaped, non-flagellated, non-motile and non-gliding bacterium, designated strain Z0201, was isolated from lake water in Yunnan, China (26° 16′ N, 99° 94′ E). Cells of strain Z0201 were 0.2–0.4 µm wide and 1.4–2.5 µm long, catalase-positive and oxidase-negative. Strain Z0201 was found to grow at 4–37 °C (optimum, 30 °C) and pH 6.5–8.5 (pH 7.5) in the presence of 0–2.0 % (w/v) NaCl (0–0.5 %). The sole respiratory quinone of strain Z0201 was MK-7 and the DNA G+C content was 41.2 mol%. The major fatty acid was iso-C15 : 0 (49.4 %). The polar lipid profile of strain Z0201 consisted of aminophospholipid, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, two unidentified phospholipids and five unidentified lipids. Based on the 16S rRNA gene sequence analysis, strain Z0201 was a member of the genus Aquiflexum , appearing to be closely related to Aquiflexum balticum (95.4 %). On the basis of phenotypic distinctiveness and phylogenetic divergence, strain Z0201 is considered to represent a novel species of the genus Aquiflexum , for which the name Aquiflexum aquatile sp. nov. is proposed. The type strain is Z0201 (=KCTC 62450=MCCC 1H00328).

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/content/journal/ijsem/10.1099/ijsem.0.003405
2019-04-30
2020-01-22
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