A Gram-stain-negative oval-rod-shaped, spore-forming anaerobic bacterium, designated as strain MCWD5T, was isolated from sediment of a salt pond in the Republic of Korea (35° 7′ 18″ N 126° 19′ 4″ E). The 16S rRNA gene sequence analysis revealed that strain MCWD5T had low similarity values to members in the family Lachnospiraceae, such as Robinsoniella peoriensis PPC31T (94.8 %), Ruminococcusgauvreauii CCRI-16110T (94.2 %) and Lachnotalea glycerini DLD10T (94.0 %), and its phylogenetic position is unstable. The strain could grow at 20–42 °C (optimum, 38–42 °C), pH 5.5–10.0 (pH 7.0) and with 0–6 % (2.0 %) NaCl. Strain MCWD5T could not use nitrate, nitrite, sulfate or sulfite as electron acceptors. The strain could utilize various carbohydrates, such as arabinose, cellobiose, glucose, etc., and polymers such as pectin and starch. The major fatty acids of strain MCWD5T were C14 : 0, C16 : 0, C16 : 1ω7c, C18 : 1ω7c DMA and summed feature 8 (C17 : 1ω8c and/or C17 : 2), which was clearly different from those of related genera. The major polar lipids were diphosphatidyglycerol, phosphatidyglycerol and an unknown phospholipid. Based on the results of phylogenetic, physiologic and chemotaxonomic studies, Anaerosacchariphilus polymeriproducens gen. nov., sp. nov. with the type strain MCWD5T (=KCTC 15595T=DSM 105757T) is proposed in the family Lachnospiraceae.
RaineyFA.
Order I. Clostridiales Prévot 1953. In
De VosP,
GarrityGM,
JonesD,
KriegNR,
LudwigW et al.
(editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 3 New York: Springer; 2009 pp. 736
MeehanCJ,
BeikoRG.
A phylogenomic view of ecological specialization in the Lachnospiraceae, a family of digestive tract-associated bacteria. Genome Biol Evol2014; 6:703–713 [View Article][PubMed]
SchouwA,
Leiknes EideT,
StokkeR,
Birger PedersenR,
Helene SteenI et al.Abyssivirga alkaniphila gen. nov., sp. nov., an alkane-degrading, anaerobic bacterium from a deep-sea hydrothermal vent system, and emended descriptions of Natranaerovirga pectinivora and Natranaerovirga hydrolytica
. Int J Syst Evol Microbiol2016; 66:1724–1734 [View Article][PubMed]
UekiA,
OhtakiY,
KakuN,
UekiK.
Descriptions of Anaerotaenia torta gen. nov., sp. nov. and Anaerocolumna cellulosilytica gen. nov., sp. nov. isolated from a methanogenic reactor of cattle waste and reclassification of Clostridium aminovalericum, Clostridium jejuense and Clostridium xylanovorans as Anaerocolumna species. Int J Syst Evol Microbiol2016; 66:2936–2943 [View Article][PubMed]
UekiA,
GotoK,
OhtakiY,
KakuN,
UekiK.
Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum
. Int J Syst Evol Microbiol2017; 67:4146–4153 [View Article][PubMed]
KoeckDE,
LudwigW,
WannerG,
ZverlovVV,
LieblW et al.Herbinix hemicellulosilytica gen. nov., sp. nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor. Int J Syst Evol Microbiol2015; 65:2365–2371 [View Article][PubMed]
JarzembowskaM,
SousaDZ,
BeyerF,
ZwijnenburgA,
PluggeCM et al.Lachnotalea glycerini gen. nov., sp. nov., an anaerobe isolated from a nanofiltration unit treating anoxic groundwater. Int J Syst Evol Microbiol2016; 66:774–779 [View Article][PubMed]
LomansBP,
LeijdekkersP,
WesselinkJJ,
BakkesP,
PolA et al. Obligate sulfide-dependent degradation of methoxylated aromatic compounds and formation of methanethiol and dimethyl sulfide by a freshwater sediment isolate, Parasporobacterium paucivorans gen. nov., sp. nov. Appl Environ Microbiol2001; 67:4017–4023 [View Article][PubMed]
CottaMA,
WhiteheadTR,
FalsenE,
MooreE,
LawsonPA.
Robinsoniella peoriensis gen. nov., sp. nov., isolated from a swine-manure storage pit and a human clinical source. Int J Syst Evol Microbiol2009; 59:150–155 [View Article][PubMed]
GiovannoniSJ.
The polymerase chain reaction. In
StackebrandtE,
GoodfellowM.
(editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: John Wiley and Sons Ltd; 1991 pp. 177–203
LeeJ-W,
KwonKK,
AziziA,
OhH-M,
KimW et al. Microbial community structures of methane hydrate-bearing sediments in the Ulleung Basin, East Sea of Korea. Mar Pet Geol2013; 47:136–146 [View Article]
YoonSH,
HaSM,
KwonS,
LimJ,
KimY et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617 [View Article][PubMed]
NaSI,
KimYO,
YoonSH,
HaSM,
BaekI et al. UBCG: Up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol2018; 56:280–285 [View Article][PubMed]
Ben HaniaW,
JosephM,
SchumannP,
BunkB,
FiebigA et al. Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat. Stand Genomic Sci2015; 10:7 [View Article][PubMed]
YangSH,
SeoHS,
OhHM,
KimSJ,
LeeJH et al.Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale. Int J Syst Evol Microbiol2013; 63:1105–1110 [View Article][PubMed]
MinnikinDE,
O'DonnellAG,
GoodfellowM,
AldersonG,
AthalyeM et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods1984; 2:233–241 [View Article]
DomingoMC,
HuletskyA,
BoissinotM,
BernardKA,
PicardFJ et al.Ruminococcus gauvreauii sp. nov., a glycopeptide-resistant species isolated from a human faecal specimen. Int J Syst Evol Microbiol2008; 58:1393–1397 [View Article][PubMed]
SakamotoM,
IinoT,
OhkumaM.
Faecalimonas umbilicata gen. nov., sp. nov., isolated from human faeces, and reclassification of Eubacterium contortum, Eubacterium fissicatena and Clostridium oroticum as Faecalicatena contorta gen. nov., comb. nov., Faecalicatena fissicatena comb. nov. and Faecalicatena orotica comb. nov. Int J Syst Evol Microbiol2017; 67:1219–1227 [View Article][PubMed]
RaineyFA,
HollenBJ,
SmallA.
Genus I. Clostridium prazmowski 1880, 23. In
De VosP,
GarrityGM,
JonesD,
KriegNR,
LudwigW et al.
(editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 3 New York: Springer; 2009 pp. 738–828
SchaalKP.
Identification of clinically significant Actinomycetes and related bacteria using chemical techniques. In
GoodfellowM,
MinnikinDE.
(editors) Chemicl Methods in Baterial Systematics London: Academic Press; 1985 pp. 359–381