RT Journal Article SR Electronic(1) A1 Zhao, Huilin A1 Zhang, Cong A1 Wu, Yulong A1 Zhang, Xiying A1 Rong, Qianyu A1 Xu, Zheng A1 Ding, Yunfei A1 Li, Yan A1 Du, Zhenzhen A1 Zhou, Xiuzhi A1 Li, Boqing A1 Ji, XiaofeiYR 2019 T1 Thalassorhabdomicrobium marinisediminis gen. nov., sp. nov., a member of the family Hyphomonadaceae isolated from the Bohai Sea JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 6 SP 1794 OP 1799 DO https://doi.org/10.1099/ijsem.0.003394 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-negative bacterium, designated strain BH-SD16T, was isolated from a marine sediment sample collected in the Bohai Sea. Cells of strain BH-SD16T are aerobic, non-flagellated oval-shaped rods, showing oxidase- and catalase-positive activities. Growth occurs between 15–45 °C (optimum, 30 °C), at pH 6.0–9.0 (pH 7.0–7.5) and with 1–10 % (w/v) NaCl (3.0 %). Strain BH-SD16T contains C18 : 1 ω7c (49.2 %), C16 : 0 (17.7 %) and C18 : 1 ω7c 11-methyl (16.6 %) as the predominant fatty acids and ubiquinone-10 as the major respiratory quinone. The major polar lipids comprise phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol and two glycolipids. The size of the draft genome is 3 442 538 bp, including 3213 protein-coding genes, 40 tRNA genes and three rRNA genes, and the DNA G+C content is 63.4 mol%. Strain BH-SD16T shows the highest 16S rRNA gene sequence similarity to Pseudooctadecabacter jejudonensis (95.7 %), strains of the genus Octadecabacter (95.4–95.6 %) and strains of the genus Loktanella (93.8–95.4 %). Phylogenetic trees based on 16S rRNA gene sequences show that strain BH-SD16T forms a distinct lineage within the family Hyphomonadaceae , which is also confirmed in the multigenic phylogenetic tree calculated by RAxML. Based on the results of phenotypic, chemotaxonomic and phylogenetic analysis, strain BH-SD16T is considered to represent a novel genus and species in the family Hyphomonadaceae , for which the name Thalassorhabdomicrobium marinisediminis gen. nov., sp. nov. is proposed. The type strain is BH-SD16T (=CCTCC AB 2017073T=KCTC 62201T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003394