Prevotella cerevisiae sp. nov., beer-spoilage obligate anaerobic bacteria isolated from brewery wastewater Free

Abstract

Two obligate anaerobic, Gram-stain-negative, non-spore-forming bacilli (strains SBC 8034 and SBC 8065) were isolated from brewery wastewater. Cells of the two strains were rod-shaped and 0.8×2–5 µm in size. Strains SBC 8034 and SBC 8065 did not grow on Columbia agar or tryptic soy agar II with 5 % sheep blood, brain–heart infusion agar or chocolate agar, but did grow on peptone–yeast–glucose agar and de Man, Rogosa and Sharpe agar using beer instead of water. The organisms produced acetic acid and succinic acid as the major metabolic end-products. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains are clearly distinct from all recognized species within the genus Prevotella , but belong to the same species (DDH=85 %). Based on 16S rRNA and hsp60 gene sequencing, along with phenotypic, chemical and biochemical properties, strains SBC 8034 and SBC 8065 were considered to represent a novel species within the genus Prevotella , for which the name Prevotella cerevisiae sp. nov. is proposed. Strain SBC 8034 (=DSM 100619=JCM 30867) is the type strain of the proposed novel species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003393
2019-04-16
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/6/1789.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003393&mimeType=html&fmt=ahah

References

  1. Nakakita Y, Takahashi T, Sugiyama H, Shigyo T, Shinotsuka K. Isolation of novel beer-spoilage bacteria from the brewery environment. J Am Soc Brew Chem 1998; 56:114–117 [View Article]
    [Google Scholar]
  2. de Man JC, Rogosa M, Sharpe ME. A medium for the cultivation of lactobacilli. J Appl Bacteriol 1960; 23:130–135 [View Article]
    [Google Scholar]
  3. Ueki A, Matsuda K, Ohtsuki C. Sulfate-reduction in the anaerobic digestion of animal waste. J Gen Appl Microbiol 1986; 32:111–123 [View Article]
    [Google Scholar]
  4. Akasaka H, Izawa T, Ueki K, Ueki A. Phylogeny of numerically abundant culturable anaerobic bacteria associated with degradation of rice plant residue in Japanese paddy field soil. FEMS Microbiol Ecol 2003; 43:149–161 [View Article][PubMed]
    [Google Scholar]
  5. Akasaka H, Ueki A, Hanada S, Kamagata Y, Ueki K. Propionicimonas paludicola gen. nov., sp. nov., a novel facultatively anaerobic, Gram-positive, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil. Int J Syst Evol Microbiol 2003; 53:1991–1998 [View Article][PubMed]
    [Google Scholar]
  6. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586[PubMed]
    [Google Scholar]
  7. Miyagawa E, Azuma R, Suto T. Cellular fatty acid composition in gram-negative obligately anaerobic rods. J Gen Appl Microbiol 1979; 25:41–51 [View Article]
    [Google Scholar]
  8. Ueki A, Suto T. Cellular fatty acid composition of sulfate-reducing bacteria. J Gen Appl Microbiol 1979; 25:185–196 [View Article]
    [Google Scholar]
  9. Moore LV, Bourne DM, Moore WE. Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. Int J Syst Bacteriol 1994; 44:338–347 [View Article][PubMed]
    [Google Scholar]
  10. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–218 [View Article]
    [Google Scholar]
  11. Saito H, Miura KI. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 1963; 72:619–629 [View Article][PubMed]
    [Google Scholar]
  12. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
    [Google Scholar]
  13. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 1989; 39:224–229
    [Google Scholar]
  14. Lane DJ. 16S/23S rRNA sequencing. Nucleic Acid Techniques in Bacterial Systematics 1991 pp. 115–175
    [Google Scholar]
  15. Sakamoto M, Ohkuma M. Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods. J Med Microbiol 2010; 59:1293–1302 [View Article][PubMed]
    [Google Scholar]
  16. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  17. Mcwilliam H, Li W, Uludag M, Squizzato S, Park YM et al. Analysis Tool Web Services from the EMBL-EBI. Nucleic Acids Res 2013; 41:W597–W600 [View Article][PubMed]
    [Google Scholar]
  18. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  20. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  21. Shah HN, Collins DM. Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides . Int J Syst Bacteriol 1990; 40:205–208 [View Article][PubMed]
    [Google Scholar]
  22. Sakamoto M, Ohkuma M. Identification and classification of the genus Bacteroides by multilocus sequence analysis. Microbiology 2011; 157:3388–3397 [View Article][PubMed]
    [Google Scholar]
  23. Avgustin G, Wallace RJ, Flint HJ. Phenotypic diversity among ruminal isolates of Prevotella ruminicola: proposal of Prevotella brevis sp. nov., Prevotella bryantii sp. nov., and Prevotella albensis sp. nov. and redefinition of Prevotella ruminicola . Int J Syst Bacteriol 1997; 47:566–568 [View Article][PubMed]
    [Google Scholar]
  24. Sakamoto M, Umeda M, Ishikawa I, Benno Y. Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque. Int J Syst Evol Microbiol 2005; 55:1839–1843 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003393
Loading
/content/journal/ijsem/10.1099/ijsem.0.003393
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed