1887

Abstract

Two obligate anaerobic, Gram-stain-negative, non-spore-forming bacilli (strains SBC 8034 and SBC 8065) were isolated from brewery wastewater. Cells of the two strains were rod-shaped and 0.8×2–5 µm in size. Strains SBC 8034 and SBC 8065 did not grow on Columbia agar or tryptic soy agar II with 5 % sheep blood, brain–heart infusion agar or chocolate agar, but did grow on peptone–yeast–glucose agar and de Man, Rogosa and Sharpe agar using beer instead of water. The organisms produced acetic acid and succinic acid as the major metabolic end-products. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains are clearly distinct from all recognized species within the genus Prevotella , but belong to the same species (DDH=85 %). Based on 16S rRNA and hsp60 gene sequencing, along with phenotypic, chemical and biochemical properties, strains SBC 8034 and SBC 8065 were considered to represent a novel species within the genus Prevotella , for which the name Prevotella cerevisiae sp. nov. is proposed. Strain SBC 8034 (=DSM 100619=JCM 30867) is the type strain of the proposed novel species.

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2019-04-16
2019-10-20
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