%0 Journal Article %A Zheng, Yu %A Wang, Chiung-Mei %A Sakai, Yasuteru %A Abe, Keietsu %A Yokota, Akira %A Yabe, Shuhei %T Thermogemmatispora aurantia sp. nov. and Thermogemmatispora argillosa sp. nov., within the class Ktedonobacteria, and emended description of the genus Thermogemmatispora %D 2019 %J International Journal of Systematic and Evolutionary Microbiology, %V 69 %N 6 %P 1744-1750 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003388 %K genus Thermogemmatispora %K Ktedonobacteria %I Microbiology Society, %X Two thermophilic, aerobic, Gram-stain-positive Ktedonobacteria strains, A1-2T and A3-2T, were isolated from geothermal soil in Japan. The strains formed orange-coloured colonies on 10-fold diluted Reasoner’s 2A medium, followed by formation of branched aerial mycelium with multiple grape-like spores. Both strains hydrolysed casein, carboxymethyl cellulose, starch, chitin and xylan, but did not liquify gelatin. Strain A1-2T utilised sucrose and gellan gum and was inhibited by inositol, while strain A3-2T utilised only gellan gum and was not inhibited by inositol. The DNA G+C contents of strain A1-2T and A3-2T were 63.2 and 63.1 mol%, respectively. Chemotaxonomic data (major fatty acid, iso-C17 : 0; major menaquinone, MK-9(H2); cell-wall amino acids, ornithine, serine, glycine, glutamic acid, alanine and β-alanine; polar lipids, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid, one unidentified phosphoglycolipid and three unidentified glycolipids; major cell-wall sugars, mannose, arabinose and xylose) indicate that both strains belong to the genus Thermogemmatispora. 16S rRNA gene sequence analysis indicated that strain A1-2 T was most closely related to the type strains of Thermogemmatispora onikobensis (97.7 % sequence similarity), and that strain A3-2T was most closely related to the type strains of Thermogemmatispora carboxidivorans(97.2%), but DNA–DNA hybridization shows relatedness values of <67 % with previously described type strains. Moreover, 16S rRNA gene sequence similarity and DNA–DNA relatedness between strain A1-2T and strain A3-2T were 96.0 and 33.4%, respectively, suggesting that the two strains are genetically distinct. The two strains are proposed as Thermogemmatispora aurantia sp. nov. and Thermogemmatispora argillosa sp. nov. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003388